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Optimizing the enzymatic synthesis of beta-D-galactopyranosyl-D-xyloses for their use in the evaluation of lactase activity in vivo.

作者信息

Hermida Carmen, Corrales Guillermo, Cañada Francisco J, Aragón Juan J, Fernández-Mayoralas Alfonso

机构信息

Departamento de Química Orgánica Biológica, Instituto de Química Orgánica General, CSIC, Juan de la Cierva 3, 28006 Madrid, Spain.

出版信息

Bioorg Med Chem. 2007 Jul 15;15(14):4836-40. doi: 10.1016/j.bmc.2007.04.067. Epub 2007 May 6.

DOI:10.1016/j.bmc.2007.04.067
PMID:17512743
Abstract

Disaccharides 2-O-, 3-O-, and 4-O-beta-D-galactopyranosyl-D-xyloses (2, 3, and 1, respectively) were obtained by beta-galactosidase-catalyzed reactions for their use in the evaluation of intestinal lactase activity in vivo. Their administration to suckling rats followed by determination of the derived D-xylose in the urine and measurement of lactase activity in intestinal homogenates showed 1 to be the most suitable disaccharide for a potential test of the deficiency of intestinal lactase. The synthesis of 1 was further studied by evaluating the effect of different variables on the yield and regioselectivity of the enzymatic galactosylation, and the purification process was optimized.

摘要

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