Klein Alexander, Metz Gerlinde A, Papazoglou Anna, Nikkhah Guido
Laboratory of Molecular Neurosurgery, Department of Stereotactic Neurosurgery, University Hospital Freiburg Neurocentre, Breisacher Str 64, Freiburg, Germany.
Neurobiol Dis. 2007 Jul;27(1):24-35. doi: 10.1016/j.nbd.2007.03.010. Epub 2007 Apr 5.
Skilled forelimb movements depend on an intact dopaminergic (DA) neurotransmission and are substantially impaired in the unilateral rat model of Parkinson's disease. It has remained unclear, however, to what extent reaching and grasping movements can be influenced by intrastriatal transplantation of fetal DA neurons. Here an extensive behavioral assessment of skilled forelimb movement patterns in hemiparkinsonian and DA-grafted rats was carried out. Good DA graft survival was accompanied by a compensation of drug-induced rotational asymmetries. Interestingly, skilled forelimb use was significantly improved in transplanted animals as compared to lesion-only animals in the staircase test. Qualitative analysis of single forelimb reaching movement components revealed dissociable patterns of graft effects: while some movement components in grafted animals improved, others remained unchanged or even deteriorated. These findings provide novel insights into the complex interactions of graft-derived restoration of DA neurotransmission and skilled forelimb behavior.
熟练的前肢运动依赖于完整的多巴胺能（DA）神经传递，并且在帕金森病的单侧大鼠模型中会受到严重损害。然而，胎儿DA神经元的纹状体内移植能在多大程度上影响伸展和抓握运动仍不清楚。在此，我们对偏侧帕金森病大鼠和DA移植大鼠的熟练前肢运动模式进行了广泛的行为评估。良好的DA移植存活伴随着药物诱导的旋转不对称性的补偿。有趣的是，与仅损伤动物相比，在阶梯试验中，移植动物的熟练前肢使用情况有显著改善。对单个前肢伸展运动成分的定性分析揭示了移植效应的可分离模式：虽然移植动物的一些运动成分有所改善，但其他成分保持不变甚至恶化。这些发现为移植衍生的DA神经传递恢复与熟练前肢行为之间的复杂相互作用提供了新的见解。
