Kuo Hsien-Wen, Chou Sze-Yuan, Hu Tsung-Wen, Wu Fang-Yang, Chen Da-Jen
Institute of Environmental Health, China Medical University, No. 91, Hsueh-Shin Road, Taichung, Taiwan.
Mutat Res. 2007 Jul 10;631(1):62-8. doi: 10.1016/j.mrgentox.2007.04.009. Epub 2007 Apr 20.
Reactive oxygen species (ROS) causes damage to DNA, but the role of ROS in breast carcinoma is still not clear. The objective of this study was to measure the urinary 8-OHdG levels of breast cancer patients at each stage of carcinogenesis and assess its association with the development of breast cancer. Sixty patients with malignant breast tumors were matched with 60 control subjects of the same ages in this case control study. Urinary 8-OHdG levels were significantly higher among breast cancer patients than among the control subjects, after making adjustments for confounders such as smoking, coffee consumption and use of oral contraceptives. The breast cancer patients were divided into three groups based on the stages of their cancer; urinary 8-OHdG levels decreased with each stage of breast carcinoma. Using multiple regression and logistic models adjusted for other covariates, urinary 8-OHdG levels significantly correlated with the development of breast cancer. However, it was found that breast cancer was not significantly influenced by CYP1A1, CYP1M1 or NAT2 polymorphisms. In conclusion, it was found that oxygen radical generation occurred within carcinoma cells, but the role of polymorphism of specific genes in the development of breast cancer should be evaluated.
活性氧（ROS）会对DNA造成损伤，但ROS在乳腺癌中的作用仍不明确。本研究的目的是测量乳腺癌患者在癌变各阶段的尿8-羟基脱氧鸟苷（8-OHdG）水平，并评估其与乳腺癌发生发展的关系。在这项病例对照研究中，60例患有恶性乳腺肿瘤的患者与60例年龄相同的对照受试者进行了匹配。在对吸烟、咖啡摄入量和口服避孕药使用等混杂因素进行调整后，乳腺癌患者的尿8-OHdG水平显著高于对照受试者。根据癌症分期，将乳腺癌患者分为三组；尿8-OHdG水平随乳腺癌分期的增加而降低。使用针对其他协变量进行调整的多元回归和逻辑模型，尿8-OHdG水平与乳腺癌的发生发展显著相关。然而，发现乳腺癌并未受到细胞色素P450 1A1（CYP1A1）、细胞色素P450 1M1（CYP1M1）或N-乙酰基转移酶2（NAT2）基因多态性的显著影响。总之，发现癌细胞内会产生活性氧，但特定基因多态性在乳腺癌发生发展中的作用仍需评估。
