高效液相色谱法测定绵羊血浆中奋乃静的含量：在药代动力学研究中的应用。

HPLC quantification of perphenazine in sheep plasma: application to a pharmacokinetic study.

作者信息

Pedernera Cecilia, Ruiz José Luis, Castells Glòria, Manteca Xavier, Cristòfol Carles

机构信息

Unitat de Fisiologia Animal, Facultat de Veterinària, UAB, Spain.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Jul 1;854(1-2):308-12. doi: 10.1016/j.jchromb.2007.04.038. Epub 2007 May 10.

DOI:10.1016/j.jchromb.2007.04.038

PMID:17512805

Abstract

A high performance liquid chromatographic (HPLC) method for the determination of perphenazine (PPZ) in sheep plasma was developed and validated. The separation was achieved using a 5 microm C18 column (125 mm x 4 mm) with a mobile phase composed of acetonitrile and an aqueous solution of H(3)PO(4) and TBA (inverse gradient). The flow rate was 1.5 mL/min and the UV detection was performed at 258 nm. The method was validated with respect to linearity, intra and inter-day precision and accuracy, limit of quantification, limit of detection and storage stability. This method was used to perform a pilot pharmacokinetic study of PPZ after subcutaneous administration to one ewe.

摘要

建立并验证了一种用于测定绵羊血浆中奋乃静（PPZ）的高效液相色谱（HPLC）方法。使用5微米的C18柱（125毫米×4毫米）进行分离，流动相由乙腈与H₃PO₄和TBA的水溶液组成（反相梯度）。流速为1.5毫升/分钟，在258纳米处进行紫外检测。该方法在线性、日内和日间精密度与准确度、定量限、检测限以及储存稳定性方面进行了验证。该方法用于对一只母羊皮下注射PPZ后的初步药代动力学研究。