Chang Cicero Lee-Tian, Chang Shu-Lin, Lee Yi-Mei, Chiang Yi-Ming, Chuang Da-Yung, Kuo Hui-Kai, Yang Wen-Chin
Agricultural Biotechnology Research Center, Academia Sinica, Taiwan, Republic of China.
J Immunol. 2007 Jun 1;178(11):6984-93. doi: 10.4049/jimmunol.178.11.6984.
Some polyacetylenes from the plant Bidens pilosa have been reported to treat diabetes. In this study, we report that the cytopiloyne from B. pilosa, which is structurally different from the above-mentioned polyacetylenes and inhibits CD4(+) T cell proliferation, effectively prevents the development of diabetes in nonobese diabetic mice as evidenced by a normal level of blood glucose and insulin and normal pancreatic islet architecture. Cytopiloyne also suppresses the differentiation of type 1 Th cells but promotes that of type 2 Th cells, which is consistent with it enhancing GATA-3 transcription. Also, long-term application of cytopiloyne significantly decreases the level of CD4(+) T cells inside pancreatic lymph nodes and spleens but does not compromise total Ab responses mediated by T cells. Coculture assays imply that this decrease in CD4(+) T cells involves the Fas ligand/Fas pathway. Overall, our results suggest that cytopiloyne prevents type 1 diabetes mainly via T cell regulation.
据报道，植物鬼针草中的一些聚乙炔类化合物可用于治疗糖尿病。在本研究中，我们报告称，鬼针草中的细胞多聚炔，其结构与上述聚乙炔类化合物不同，可抑制CD4(+) T细胞增殖，能有效预防非肥胖糖尿病小鼠糖尿病的发展，血糖和胰岛素水平正常以及胰岛结构正常即可证明这一点。细胞多聚炔还可抑制1型Th细胞的分化，但促进2型Th细胞的分化，这与其增强GATA-3转录一致。此外，长期应用细胞多聚炔可显著降低胰腺淋巴结和脾脏内CD4(+) T细胞的水平，但不会损害T细胞介导的总抗体反应。共培养试验表明，CD4(+) T细胞的这种减少涉及Fas配体/Fas途径。总体而言，我们的结果表明，细胞多聚炔主要通过调节T细胞来预防1型糖尿病。
