Langkilde N C, Wolf H, Orntoft T F
Department of Urology, Skejby Hospital, Aarhus, Denmark.
Br J Haematol. 1991 Nov;79(3):493-9. doi: 10.1111/j.1365-2141.1991.tb08061.x.
Eight red blood cell (RBC) Le(a-b-) individuals were selected from a series of patients with bladder or colon cancer. Defined by the presence or absence of alpha 1-4-L-fucosyltransferase activity in saliva, four of these patients were characterized as non-genuine Lewis negative [RBC Le(a-b-) with alpha 1-4-L-fucosyltransferase activity in saliva], and four as genuine Lewis negative [RBC Le(a-b-) with no alpha 1-4-L-fucosyltransferase activity in saliva]. Stainings of paraffin embedded formalin fixed tissue sections for Lea and Leb antigens were performed by means of an indirect immunohistochemical method on all malignant and benign tissue previously removed from these eight patients. Leb antigens were always expressed independently of both the Lewis and the secretor status of the individual. Lea antigens, on the other hand, showed a different staining pattern. Although primarily expressed in non-genuine Le(a-b-) individuals, Lea antigens were expressed in genuine Le(a-b-) individuals as well--to a limited extent, but still detectable. Thus, these findings seem to show that the Lewis antigen expression is tissue dependent, and it is not possible to predict tissue Lewis antigen expression by merely examining erythrocytes or saliva.
从一系列膀胱癌或结肠癌患者中挑选出8名红细胞Le(a-b-)个体。根据唾液中α1-4-L-岩藻糖基转移酶活性的有无来定义，其中4名患者被表征为非真正的Lewis阴性（唾液中有α1-4-L-岩藻糖基转移酶活性的红细胞Le(a-b-)），另外4名则为真正的Lewis阴性（唾液中无α1-4-L-岩藻糖基转移酶活性的红细胞Le(a-b-)）。通过间接免疫组化方法对从这8名患者先前切除的所有恶性和良性组织的石蜡包埋福尔马林固定组织切片进行Lea和Leb抗原染色。Leb抗原的表达总是独立于个体的Lewis和分泌状态。另一方面，Lea抗原显示出不同的染色模式。虽然主要在非真正的Le(a-b-)个体中表达，但Lea抗原在真正的Le(a-b-)个体中也有表达——程度有限，但仍可检测到。因此，这些发现似乎表明Lewis抗原的表达是组织依赖性的，仅通过检查红细胞或唾液无法预测组织Lewis抗原的表达。
