Takehara Kyoko, Kubushiro Kaneyuki, Kiguchi Kazushige, Ishiwata Isamu, Tsukazaki Katsumi, Nozawa Shiro, Iwamori Masao
Department Of Obstetrics and Gynecology, School of Medicine, Keio University, Tokyo, Japan.
Jpn J Cancer Res. 2002 Oct;93(10):1129-37. doi: 10.1111/j.1349-7006.2002.tb01215.x.
Transformation-associated expression of Le(b) (Lewis antigen-b) or Le(Y) in human colorectal carcinomas has been well described. To examine the expression of glycosphingolipids (GSLs) bearing Lewis-phenotypes in human gynecological carcinoma-derived cells, we determined the concentrations of all GSLs. Although neither Le(b) nor Le(Y) was present in HEC-108 cells established from the poorly differentiated type of endometrial adenocarcinoma, other cell lines from moderately or well-differentiated types expressed either Le(b) or Le(Y), or both, at concentrations of 0.01 to 0.03 microg per mg of dry cells, which comprised 0.3 to 1.3% of the total GSLs. In the cervical and ovarian carcinoma-derived cell lines, Lewis phenotypes tended to be carried by nLc(4)Cer, which was accumulated in the cells without sialylation or fucosylation. These results indicated that expression of Le(b)- or Le(Y)-phenotypes was strongly dependent on the metabolic ability to supply the precursor GSLs. Both Le(b) and Le(Y) were successfully detected by monoclonal antibody MSN-1, which was a useful probe for the simultaneous detection of Le(b) and Le(Y). On application of MSN-1, either Le(b) or Le(Y) was detected in tissues from patients with well- and moderately differentiated types of endometrial adenocarcinoma at concentrations of 0.01 to 0.04 microg per mg of dry tissues, but not in the tissues of poorly differentiated type. Normal endometria at the follicular and luteal phases also contained the antigens, but the concentrations and the frequency of antigen expression were lower than those in the well- and moderately differentiated types of endometrial adenocarcinoma.
人类结直肠癌中Le(b)（刘易斯抗原-b）或Le(Y)的转化相关表达已得到充分描述。为了检测人类妇科癌衍生细胞中携带刘易斯表型的糖鞘脂（GSLs）的表达，我们测定了所有GSLs的浓度。虽然从低分化型子宫内膜腺癌建立的HEC-108细胞中既不存在Le(b)也不存在Le(Y)，但来自中分化或高分化型的其他细胞系以每毫克干细胞0.01至0.03微克的浓度表达Le(b)或Le(Y)，或两者都表达，这占总GSLs的0.3%至1.3%。在宫颈癌和卵巢癌衍生的细胞系中，刘易斯表型倾向于由nLc(4)Cer携带，其在细胞中积累而没有唾液酸化或岩藻糖基化。这些结果表明，Le(b)或Le(Y)表型的表达强烈依赖于供应前体GSLs的代谢能力。单克隆抗体MSN-1成功检测到了Le(b)和Le(Y)，它是同时检测Le(b)和Le(Y)的有用探针。应用MSN-1时，在高分化和中分化型子宫内膜腺癌患者的组织中检测到Le(b)或Le(Y)，浓度为每毫克干组织0.01至0.04微克，但在低分化型组织中未检测到。卵泡期和黄体期的正常子宫内膜也含有这些抗原，但抗原表达的浓度和频率低于高分化和中分化型子宫内膜腺癌。
