Tada Junji, Kono Taiki, Suda Akira, Mizuno Hideaki, Miyawaki Atsushi, Midorikawa Katsumi, Kannari Fumihiko
Department of Electronics and Electrical Engineering, Keio University, 3-14-1, Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan.
Appl Opt. 2007 May 20;46(15):3023-30. doi: 10.1364/ao.46.003023.
Selective fluorescence excitation of specific molecular species is demonstrated by using coherent control of two-photon excitation with supercontinuum pulses generated with a microstructure fiber. Pulse shaping prior to pulse propagation through the fiber is controlled by a self-learning optimization loop so that the highest fluorescence signal contrast between two fluorescent proteins is obtainable. The self-learning optimization loop successfully controls both the optical nonlinarity of the microstructure fiber and the two-photon excitation of the fluorescent proteins.
