可变外显子的使用选择性地决定了酰基辅酶A硫酯酶7基因产物的组织分布和亚细胞定位。
Alternative exon usage selectively determines both tissue distribution and subcellular localization of the acyl-CoA thioesterase 7 gene products.
作者信息
Hunt M C, Greene S, Hultenby K, Svensson L T, Engberg S, Alexson S E H
机构信息
Department of Laboratory Medicine, Division of Clinical Chemistry C1- 74, Karolinska Institutet, Karolinska University Hospital at Huddinge, Stockholm, Sweden.
出版信息
Cell Mol Life Sci. 2007 Jun;64(12):1558-70. doi: 10.1007/s00018-007-7062-6.
Acyl-CoA thioesterases (ACOTs) catalyze the hydrolysis of acyl-CoAs to free fatty acids and coenzyme A. Recent studies have demonstrated that one gene named Acot7, reported to be mainly expressed in brain and testis, is transcribed in several different isoforms by alternative usage of first exons. Strongly decreased levels of ACOT7 activity and protein in both mitochondria and cytosol was reported in patients diagnosed with fatty acid oxidation defects, linking ACOT7 function to regulation of fatty acid oxidation in other tissues. In this study, we have identified five possible first exons in mouse Acot7 (Acot7a-e) and show that all five first exons are transcribed in a tissue-specific manner. Taken together, these data show that the Acot7 gene is expressed as multiple isoforms in a tissue-specific manner, and that expression in tissues other than brain and testis is likely to play important roles in fatty acid metabolism.
酰基辅酶A硫酯酶（ACOTs）催化酰基辅酶A水解为游离脂肪酸和辅酶A。最近的研究表明，一个名为Acot7的基因，据报道主要在大脑和睾丸中表达，通过第一个外显子的可变使用转录出几种不同的异构体。据报道，被诊断为脂肪酸氧化缺陷的患者线粒体和细胞质中ACOT7活性和蛋白质水平均显著降低，这将ACOT7的功能与其他组织中脂肪酸氧化的调节联系起来。在本研究中，我们在小鼠Acot7中鉴定出五个可能的第一个外显子（Acot7a - e），并表明所有五个第一个外显子均以组织特异性方式转录。综上所述，这些数据表明Acot7基因以组织特异性方式表达为多种异构体，并且在大脑和睾丸以外的组织中的表达可能在脂肪酸代谢中起重要作用。
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