Jiang Hui-Yong, Zhang Xue-Feng, Liu Li, Li Hui-Ling, Zhao Tong
Department of General Surgery, Northern Hospital, No 83, Wenhua Road, Shenhe District, Shenyang, Liaoning Province, China.
In Vitro Cell Dev Biol Anim. 2007 Mar-Apr;43(3-4):109-12. doi: 10.1007/s11626-007-9019-3. Epub 2007 May 21.
Tissue microarrays are ordered arrays of hundreds to thousands of tissue cores in a single paraffin block. We invented a novel method to make a high-throughput microarray group. Conventional smaller tissue microarrays were made first and then sectioned. Separate paraffin films were arrayed orderly onto a regular-sized glass slide to form a larger microarray group. Sections were not floated in a water bath but, rather, were cut singly using conventional microtome, arrayed orderly onto the glass slide with forceps instead of using a tape-based tissue transfer system, and then unfolded with warm water (46 degrees C) using a micropipette. This not only lowers the difficulty in sectioning but the overall tissue disks can be included in the same section. A microarray group of 2,534 small disks (theoretically, 2,560 disks can be made; 26 fell off during the procedure), the most up to now, was successfully made and may be used in immunohistochemistry, mRNA in situ hybridization, and flourescent in situ hybridization.
组织微阵列是在单个石蜡块中由数百到数千个组织芯组成的有序阵列。我们发明了一种制作高通量微阵列组的新方法。首先制作传统的较小组织微阵列，然后进行切片。将单独的石蜡膜有序排列在常规尺寸的载玻片上，形成一个更大的微阵列组。切片不是在水浴中漂浮，而是使用传统切片机单独切割，用镊子而不是基于胶带的组织转移系统将其有序排列在载玻片上，然后用微量移液器用温水（46摄氏度）展开。这不仅降低了切片难度，而且整个组织盘可以包含在同一切片中。成功制作了一个由2534个小圆盘组成的微阵列组（理论上可制作2560个圆盘；过程中有26个脱落），这是目前为止最多的，可用于免疫组织化学、mRNA原位杂交和荧光原位杂交。
