Jiang Hui-yong, Zhang San-quan, Zhao Tong
Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China.
Diagn Mol Pathol. 2006 Jun;15(2):109-14. doi: 10.1097/00019606-200606000-00008.
We invented a new method to make microarrays using nuclei extracted from paraffin-embedded tissues or cultured cells. A blank recipient paraffin block with 10 x 10 cores was constructed and sectioned to make the mold for the cell arrays. The sections of paraffin were mounted on poly-L-lysine-coated slides. Prepared nuclei or cells were injected into the cores of the paraffin mold. The slides were dried and dewaxed and nuclei or cell arrays were made. Using this method, we successfully made microarrays of nuclei extracted from diffuse large B-cell lymphoma paraffin-embedded tissues, nasopharyngeal cancer and lymphoma cell lines. This technique resulted in a paraffin-embedded cell preparation that yielded a cell density of approximately 500 to 1000 or 800 cells on average per 0.6-mm-diameter core. The microarrays were successfully used in fluorescence in situ hybridization, mRNA in situ hybridization, and cytohistochemical staining.
我们发明了一种利用从石蜡包埋组织或培养细胞中提取的细胞核制作微阵列的新方法。构建了一个带有10×10个芯的空白受体石蜡块，并进行切片以制作细胞阵列模具。将石蜡切片安装在涂有聚-L-赖氨酸的载玻片上。将制备好的细胞核或细胞注入石蜡模具的芯中。将载玻片干燥、脱蜡，制成细胞核或细胞阵列。使用这种方法，我们成功地制作了从弥漫性大B细胞淋巴瘤石蜡包埋组织、鼻咽癌和淋巴瘤细胞系中提取的细胞核微阵列。该技术制备出的石蜡包埋细胞制剂，平均每个直径0.6毫米的芯中细胞密度约为500至1000个或800个细胞。这些微阵列成功应用于荧光原位杂交、mRNA原位杂交和细胞组织化学染色。
