Drewa Tomasz
Department of Tissue Engineering, Copernicus University, Bydgoszcz, Poland.
Acta Pol Pharm. 2006 Mar-Apr;63(2):153-5.
The etiology of the interstitial cystitis (IC) is believed to be a dysfunction of the bladder glycosaminoglycan (GAG) protective layer of the urothelial cells. Management of interstitial cystitis (IC) is pure empirical. The aim of this study was to establish primary rabbit urothelial cells (PRUCC) affinity to complex sugar scaffold in vitro. The primary rabbit urothelial cells culture (PRUCC) was set up. The cells were cultured in Jouan IG 150 incubator in temperature of 370C and humidified atmosphere containing 5% of CO2. The multi-well culture plates were covered with potato starch. PRUCC was seeded on the starch scaffold. On the 7th day the starch was removed. Culture analysis and photo documentation was done under inverted microscope. There was no disruption of the monolayer under the starch and the cells morphology was comparable to the rabbit urothelial cells in culture. No inhibitory effect on the PRUCC was observed. The colonies of the urothelial cells were formed over the starch grains. The athor postulate that urothelial cells have affinity to the complex sugar compounds. There is strong evidence that sugar complex compounds could be used in the treatment of the IC.
间质性膀胱炎（IC）的病因被认为是膀胱尿路上皮细胞糖胺聚糖（GAG）保护层功能障碍。间质性膀胱炎（IC）的治疗完全是经验性的。本研究的目的是在体外建立原代兔尿路上皮细胞（PRUCC）对复合糖支架的亲和力。建立了原代兔尿路上皮细胞培养（PRUCC）。细胞在Jouan IG 150培养箱中于37℃、含5%二氧化碳的湿润气氛中培养。多孔培养板覆盖有马铃薯淀粉。将PRUCC接种在淀粉支架上。第7天去除淀粉。在倒置显微镜下进行培养分析和拍照记录。淀粉下的单层细胞没有破坏，细胞形态与培养中的兔尿路上皮细胞相当。未观察到对PRUCC的抑制作用。尿路上皮细胞集落在淀粉颗粒上形成。作者推测尿路上皮细胞对复合糖化合物有亲和力。有强有力的证据表明复合糖化合物可用于治疗IC。
