Effect of continuous bioartificial kidney therapy on porcine multiple organ dysfunction syndrome with acute renal failure.

The bioartificial kidney (BK) was fabricated with primary culture of human proximal tubular cells growing in a hemofilter. A porcine model with multiple organ dysfunction syndrome, including acute renal failure, was induced by cecal ligation and puncture and bilateral ureteral ligation. The models were treated with BK (group A) or sham BK (group B) or without treatment (group C). Mean arterial pressure (mm Hg) was higher in group A (88.13 +/- 7.62) than in groups B and C (63.50 +/- 11.82, 53.50 +/- 2.52) at 24 hours (p < 0.01). Serum blood urea nitrogen, Cr, K, Pao2, and HCO3 were similar during the treatment periods between groups A and B, which were better than those in group C. In group A, tumor necrosis factor (TNF)-alpha (pg/mL) was 394.42 +/- 35.62 at 24 hours, lower than that of groups B and C (531.76 +/- 30.23, 552.89 +/- 27.81) (p < 0.05). Peak level of interleukin (IL)-10 (pg/mL) was higher in group A (272.36 +/- 48.89) than in groups B and C (106.30 +/- 29.69, 102.59 +/- 10.21) (p < 0.01). There was no difference of serum IL-6 between pretreatment and post-treatment in groups A and B, but serum IL-6 gradually increased in group C. The survival time (hours) was longer in group A than in other groups, which was prolonged by 46.20% and 58.39%. Results indicate that BK can ameliorate mean arterial pressure, decrease serum TNF-alpha, increase serum IL-10, and prolong survival time of pigs with multiple organ dysfunction syndrome and acute renal failure.