Huijuan Mao, Xiaoyun Wang, Xumin Ying, Hengjin Wang, Xia Shen
Department of Nephrology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
ASAIO J. 2007 May-Jun;53(3):329-34. doi: 10.1097/MAT.0b013e3180590be5.
The bioartificial kidney (BK) was fabricated with primary culture of human proximal tubular cells growing in a hemofilter. A porcine model with multiple organ dysfunction syndrome, including acute renal failure, was induced by cecal ligation and puncture and bilateral ureteral ligation. The models were treated with BK (group A) or sham BK (group B) or without treatment (group C). Mean arterial pressure (mm Hg) was higher in group A (88.13 +/- 7.62) than in groups B and C (63.50 +/- 11.82, 53.50 +/- 2.52) at 24 hours (p < 0.01). Serum blood urea nitrogen, Cr, K, Pao2, and HCO3 were similar during the treatment periods between groups A and B, which were better than those in group C. In group A, tumor necrosis factor (TNF)-alpha (pg/mL) was 394.42 +/- 35.62 at 24 hours, lower than that of groups B and C (531.76 +/- 30.23, 552.89 +/- 27.81) (p < 0.05). Peak level of interleukin (IL)-10 (pg/mL) was higher in group A (272.36 +/- 48.89) than in groups B and C (106.30 +/- 29.69, 102.59 +/- 10.21) (p < 0.01). There was no difference of serum IL-6 between pretreatment and post-treatment in groups A and B, but serum IL-6 gradually increased in group C. The survival time (hours) was longer in group A than in other groups, which was prolonged by 46.20% and 58.39%. Results indicate that BK can ameliorate mean arterial pressure, decrease serum TNF-alpha, increase serum IL-10, and prolong survival time of pigs with multiple organ dysfunction syndrome and acute renal failure.
生物人工肾（BK）是通过在血液滤过器中培养人近端肾小管细胞的原代培养物构建而成。通过盲肠结扎和穿刺以及双侧输尿管结扎诱导出患有包括急性肾衰竭在内的多器官功能障碍综合征的猪模型。这些模型分别接受BK治疗（A组）、假BK治疗（B组）或不接受治疗（C组）。24小时时，A组的平均动脉压（mmHg）（88.13±7.62）高于B组和C组（63.50±11.82，53.50±2.52）（p<0.01）。治疗期间，A组和B组的血清血尿素氮、肌酐、钾、动脉血氧分压和碳酸氢根相似，均优于C组。在A组中，24小时时肿瘤坏死因子（TNF）-α（pg/mL）为394.42±35.62，低于B组和C组（531.76±30.23，552.89±27.81）（p<0.05）。A组白细胞介素（IL）-10的峰值水平（pg/mL）（272.36±48.89）高于B组和C组（106.30±29.69，102.59±10.21）（p<0.01）。A组和B组治疗前和治疗后血清IL-6无差异，但C组血清IL-6逐渐升高。A组的存活时间（小时）比其他组更长，延长了46.20%和58.39%。结果表明，BK可改善平均动脉压，降低血清TNF-α，升高血清IL-10，并延长多器官功能障碍综合征和急性肾衰竭猪的存活时间。
