Sakamoto Tatsunori, Ito Juichi, Ladher Raj K
Lab for Sensory Development, RIKEN Center for Developmental Biology, Chuo-ku, Kobe, Japan.
Neuroreport. 2007 Jun 11;18(9):841-4. doi: 10.1097/WNR.0b013e3280c1e359.
Tissue culture is a standard method to study tissue interactions during embryogenesis. The serum that is usually used in culture can, however, confound results as it includes unidentified factors. In this study, we used a serum-free otocyst culture to investigate the tissue interactions that determine hair cell fate in mice otocysts. Otocysts cultured with surrounding tissues have the ability to produce mature hair cells in serum-free otocyst culture. Although isolated otocysts from E9.5 mice produced hair cells, those from E9.0 mice could not. This indicates that the mouse otocyst gains the ability to generate hair cells between E9.0 and E9.5 and that this ability depends on signals from the surrounding mesenchyme and/or the hindbrain.
组织培养是研究胚胎发育过程中组织相互作用的标准方法。然而，培养中通常使用的血清可能会混淆结果，因为它包含未明确的因子。在本研究中，我们使用无血清耳囊培养来研究决定小鼠耳囊中毛细胞命运的组织相互作用。在无血清耳囊培养中，与周围组织一起培养的耳囊有能力产生成熟的毛细胞。虽然从E9.5小鼠分离的耳囊能产生毛细胞，但从E9.0小鼠分离的耳囊则不能。这表明小鼠耳囊在E9.0到E9.5之间获得了产生毛细胞的能力，并且这种能力依赖于来自周围间充质和/或后脑的信号。
