Cheng Yuxiang, Long Mei
Alkali Soil Natural Environmental Science Center, Stress Molecular Biology Laboratory, Northeast Forestry University, Harbin, PR China.
Biotechnol Lett. 2007 Jul;29(7):1129-34. doi: 10.1007/s10529-007-9347-0. Epub 2007 Mar 20.
NADP-malic enzyme (NADP-ME, EC 1.1.1.40) functions in many different pathways in plant and may be involved in plant defense such as wound and UV-B radiation. Here, expression of the gene encoding cytosolic NADP-ME (cytoNADP-ME, GenBank Accession No. AY444338) in rice (Oryza sativa L.) seedlings was induced by salt stress (NaCl). NADP-ME activities in leaves and roots of rice also increased in response to NaCl. Transgenic Arabidopsis plants over-expressing rice cytoNADP-ME had a greater salt tolerance at the seedling stage than wild-type plants in MS medium-supplemented with different levels of NaCl. Cytosolic NADPH/NADP(+) concentration ratio of transgenic plants was higher than those of wild-type plants. These results suggest that rice cytoNADP-ME confers salt tolerance in transgenic Arabidopsis seedlings.
NADP-苹果酸酶（NADP-ME，EC 1.1.1.40）在植物的许多不同途径中发挥作用，可能参与植物防御，如伤口和紫外线-B辐射。在此，水稻（Oryza sativa L.）幼苗中编码胞质NADP-ME（cytoNADP-ME，GenBank登录号AY444338）的基因表达受盐胁迫（NaCl）诱导。水稻叶片和根中的NADP-ME活性也随NaCl处理而增加。在添加不同水平NaCl的MS培养基中，过表达水稻cytoNADP-ME的转基因拟南芥植株在幼苗期比野生型植株具有更强的耐盐性。转基因植株的胞质NADPH/NADP(+)浓度比高于野生型植株。这些结果表明，水稻cytoNADP-ME赋予转基因拟南芥幼苗耐盐性。
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