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NADP-苹果酸酶OsNADP-ME2通过参与水稻赤霉素信号传导调控株高

NADP-malic Enzyme OsNADP-ME2 Modulates Plant Height Involving in Gibberellin Signaling in Rice.

作者信息

Li Bing, Zhou Xiaolong, Yao Wei, Lin Jinjun, Ding Xiaowen, Chen Qianru, Huang Hao, Chen Wenfeng, Huang Xilai, Pan Sujun, Xiao Yinghui, Liu Jianfeng, Liu Xionglun, Liu Jinling

机构信息

Hunan Provincial Key Laboratory of Crop Gene Engineering, College of Agronomy, Hunan Agricultural University, Changsha, 410128, China.

出版信息

Rice (N Y). 2024 Aug 17;17(1):52. doi: 10.1186/s12284-024-00729-5.

DOI:10.1186/s12284-024-00729-5
PMID:39152344
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11329442/
Abstract

Plants NADP-malic enzymes (NADP-MEs) act as a class of oxidative decarboxylase to mediate malic acid metabolism in organisms. Despite NADP-MEs have been demonstrated to play pivotal roles in regulating diverse biological processes, the role of NADP-MEs involving in plant growth and development remains rarely known. Here, we characterized the function of rice cytosolic OsNADP-ME2 in regulating plant height. The results showed that RNAi silencing and knock-out of OsNADP-ME2 in rice results in a dwarf plant structure, associating with significant expression inhibition of genes involving in phytohormone Gibberellin (GA) biosynthesis and signaling transduction, but with up-regulation for the expression of GA signaling suppressor SLR1. The accumulation of major bioactive GA, GA and GA are evidently altered in RNAi lines, and exogenous GA treatment compromises the dwarf phenotype of OsNADP-ME2 RNAi lines. RNAi silencing of OsNADP-ME2 also causes the reduction of NADP-ME activity associating with decreased production of pyruvate. Thus, our data revealed a novel function of plant NADP-MEs in modulation of rice plant height through regulating bioactive GAs accumulation and GA signaling, and provided a valuable gene resource for rice plant architecture improvement.

摘要

植物NADP-苹果酸酶（NADP-MEs）作为一类氧化脱羧酶，介导生物体中的苹果酸代谢。尽管NADP-MEs已被证明在调节多种生物过程中起关键作用，但NADP-MEs在植物生长发育中的作用仍鲜为人知。在此，我们对水稻胞质OsNADP-ME2在调节株高方面的功能进行了表征。结果表明，水稻中OsNADP-ME2的RNA干扰沉默和敲除导致植株矮小，这与参与植物激素赤霉素（GA）生物合成和信号转导的基因的显著表达抑制相关，但GA信号抑制因子SLR1的表达上调。RNA干扰株系中主要生物活性GA、GA₁和GA₄的积累明显改变，外源GA处理可减轻OsNADP-ME2 RNA干扰株系的矮化表型。OsNADP-ME2的RNA干扰沉默还导致NADP-ME活性降低，同时丙酮酸产量减少。因此，我们的数据揭示了植物NADP-MEs通过调节生物活性GA积累和GA信号传导来调节水稻株高的新功能，并为改善水稻株型提供了宝贵的基因资源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01e3/11329442/c70f1b204ec8/12284_2024_729_Fig7_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01e3/11329442/c70f1b204ec8/12284_2024_729_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01e3/11329442/8f4cb041e526/12284_2024_729_Fig1_HTML.jpg
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