Sveshnikov Dmitry, Funk Christiane, Schröder Wolfgang P
Department of Chemistry, Umeå University, 90187 Umeå, Sweden.
Photosynth Res. 2007 Jul-Sep;93(1-3):101-9. doi: 10.1007/s11120-007-9171-3. Epub 2007 May 22.
The PsbP-like protein of the cyanobacterium Synechocystis sp. PCC 6803 is a peripheral component of Photosystem II, located at the lumenal side of the thylakoid membrane. Removal of this protein leads to decreased competitive potential of a PsbP-like deletion mutant when grown in a mixture with wild-type cells. Flash-induced oxygen evolution traces of the mutant show a higher probability of misses, correlated with increased amplitudes of the S-states decay in the dark. Thermoluminescence emission traces demonstrate a changed charge recombination pattern in the mutant, the S(3)Q(B)(-) couple becoming the major species instead of the S(2)Q(B)(-). Our data suggest a possible role of the PsbP-like protein in stabilisation of the charge separation in Photosystem II of cyanobacteria through interaction with the Mn cluster.
集胞藻6803（Synechocystis sp. PCC 6803）中的类PsbP蛋白是光系统II的外周成分，位于类囊体膜的腔侧。去除该蛋白会导致类PsbP缺失突变体在与野生型细胞混合培养时竞争潜力下降。该突变体的闪光诱导氧释放曲线显示漏失概率更高，这与黑暗中S态衰减幅度增加相关。热发光发射曲线表明该突变体的电荷复合模式发生了变化，S(3)Q(B)(-) 对成为主要形式，而非S(2)Q(B)(-)。我们的数据表明，类PsbP蛋白可能通过与锰簇相互作用，在稳定蓝藻光系统II中的电荷分离方面发挥作用。
