采用Flash Master Personal系统在正相硅胶上对唐古特大黄中的羟基蒽醌进行制备分离和纯化。
Preparative isolation and purification of hydroxyanthraquinones from Rheum tanguticum Maxim. on normal phase silica gel: using a Flash Master Personal system.
作者信息
Zhang Yong-Zhi, Lu Yan-Hua, Wei Dong-Zhi, Chou Gui-Xin, Zhu En-Yuan
机构信息
State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai. PR China.
出版信息
Prep Biochem Biotechnol. 2007;37(3):185-93. doi: 10.1080/10826060701386661.
A flash chromatography method for preparative separation and purification of five hydroxyanthraquinones from the Chinese medicinal herb Rheum tanguticum Maxim. ex Balf. was developed by using Flash Master Personal(+) systems. The purities of the products, chrysophanol, physcion, emodin, aloe-emodin, and rhein were all over 90%, determined by high performance liquid chromatography (HPLC). Their structures were ascertained by EI-MS, (1)H- and (13)C-NMR data, and by co-TLC and HPLC with the authentic samples available in our laboratory.
采用Flash Master Personal(+)系统开发了一种快速色谱法，用于从中药材唐古特大黄（Rheum tanguticum Maxim. ex Balf.）中制备分离和纯化5种羟基蒽醌。通过高效液相色谱（HPLC）测定，产物大黄酚、大黄素甲醚、大黄素、芦荟大黄素和大黄酸的纯度均超过90%。通过电子轰击质谱（EI-MS）、氢核磁共振（¹H-NMR）和碳核磁共振（¹³C-NMR）数据，以及与本实验室现有的标准品进行共薄层色谱（co-TLC）和HPLC分析，确定了它们的结构。
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