Potential therapeutic clue of skin-derived progenitor cells following cytokine-mediated signal overexpressed in injured spinal cord.

Recent evidence indicates that neural progenitor cell characters can be found in the population of adult skin-derived progenitor cells (SPCs). They have the ability to proliferate actively in vitro as spheres in suspension and they contain neural stem cells and several chemokines. Spheres derived from adult skin tissues have a higher capacity to differentiate into neurons in vitro. We report here that intravenous infusion of SPCs from adult skin ameliorated spinal cord lesions and improved motor function in laboratory mice with a spinal cord injury (SCI). After 4-5 weeks, transplanted SPCs survived and migrated into the injured region of the SCI very efficiently, and migrated cells were partially differentiated into neurons and glial cells. Behavioral and ultrastructural tissue analysis revealed that locomotor functions and remyelinated tissue lesions of SPCs engrafted onto SCI mice were restored significantly compared to those of the control group. Efficient migration of SPCs into SCI lesions suggests that SCI-induced chemotaxic factors facilitate the migration of SPCs. Also, we verified that SCI-induced chemotaxic factors play an important role in proliferation, migration, and differentiation of engrafted SPCs. In transplantation paradigms, the interaction between the SCI microenvironment and engrafted cells will be very important in promoting host injury repair through the induction of cell migration, proliferation, and differentiation. Finally, adult SPCs can behave as a multipotent population, suggesting potential clinical applications for SCI therapy.