Parr A M, Kulbatski I, Zahir T, Wang X, Yue C, Keating A, Tator C H
Toronto Western Research Institute, Toronto Western Hospital, 399 Bathurst Street, Toronto, ON, Canada M5T 2S8.
Neuroscience. 2008 Aug 26;155(3):760-70. doi: 10.1016/j.neuroscience.2008.05.042. Epub 2008 Jun 5.
We examined the effect of spinal cord-derived neural stem/progenitor cells (NSPCs) after delayed transplantation into the injured adult rat spinal cord with or without earlier transplantation of bone marrow-derived mesenchymal stromal cells (BMSCs). Either BMSCs or culture medium were transplanted immediately after clip compression injury (27 g force), and then, 9 days after injury, NSPCs or culture medium were transplanted. Cell survival and differentiation, functional recovery, retrograde axonal tracing, and immunoelectron microscopy were assessed. A significant improvement in functional recovery based on three different measures was seen only in the group receiving NSPCs without BMSCs, and the improved recovery was evident within 1 week of transplantation. In this group, NSPCs differentiated mainly into oligodendrocytes and astrocytes, there was ensheathing of axons at the injury site by transplanted NSPCs, an increase in host oligodendrocytes, and a trend toward an increase in retrogradely labeled supraspinal nuclei. Transplantation of the BMSC scaffold resulted in a trend toward improved survival of the NSPCs, but there was no increase in function. Thus, transplantation of adult rat NSPCs produced significant early functional improvement after spinal cord injury, suggesting an early neuroprotective action associated with oligodendrocyte survival and axonal ensheathment by transplanted NSPCs.
我们研究了脊髓来源的神经干细胞/祖细胞（NSPCs）在延迟移植到成年大鼠脊髓损伤部位后的效果，移植时伴有或不伴有早期移植的骨髓间充质基质细胞（BMSCs）。在夹伤压迫损伤（27克力）后立即移植BMSCs或培养基，然后在损伤后9天移植NSPCs或培养基。评估细胞存活与分化、功能恢复、逆行轴突追踪和免疫电子显微镜检查。仅在未接受BMSCs而接受NSPCs的组中，基于三种不同测量方法观察到功能恢复有显著改善，且在移植后1周内恢复改善明显。在该组中，NSPCs主要分化为少突胶质细胞和星形胶质细胞，移植的NSPCs在损伤部位包裹轴突，宿主少突胶质细胞增加，并且逆行标记的脊髓上核有增加的趋势。BMSC支架移植使NSPCs存活有改善的趋势，但功能没有增加。因此，成年大鼠NSPCs移植在脊髓损伤后产生了显著的早期功能改善，提示与移植的NSPCs的少突胶质细胞存活和轴突包裹相关的早期神经保护作用。
