Frederick J, Buck M E, Matson D J, Cortright D N
Western Connecticut State University, 181 White Street, Danbury, CT 06810, USA.
Biochem Biophys Res Commun. 2007 Jul 13;358(4):1058-64. doi: 10.1016/j.bbrc.2007.05.029. Epub 2007 May 14.
Thermosensitive TRP channels display unique thermal responses, suggesting distinct roles mediating sensory transmission of temperature. However, whether relative expression of these channels in dorsal root ganglia (DRG) is altered in nerve injury is unknown. We developed a multiplex ribonuclease protection assay (RPA) to quantify rat TRPV1, TRPV2, TRPV3, TRPV4, TRPA1, and TRPM8 RNA levels in DRG. We used the multiplex RPA to measure thermosensitive TRP channel RNA levels in DRG from RTX-treated rats (300 microg/kg) or rats with unilateral sciatic nerve chronic constriction injury (CCI). TRPV1 and TRPA1 RNA were significantly decreased in DRG from RTX-treated rats, indicating functional colocalization of TRPA1 and TRPV1 in sensory nociceptors. In DRG from CCI rats, TRPA1, TRPV2, and TRPM8 RNA showed slight but significant increases ipsilateral to peripheral nerve injury. Our findings support the hypothesis that increased TRP channel expression in sensory neurons may contribute to mechanical and cold hypersensitivity.
热敏性瞬时受体电位（TRP）通道表现出独特的热反应，这表明它们在介导温度感觉传递中具有不同的作用。然而，这些通道在背根神经节（DRG）中的相对表达在神经损伤时是否发生改变尚不清楚。我们开发了一种多重核糖核酸酶保护分析（RPA）方法来定量大鼠DRG中TRPV1、TRPV2、TRPV3、TRPV4、TRPA1和TRPM8的RNA水平。我们使用多重RPA来测量来自经RTX处理的大鼠（300微克/千克）或单侧坐骨神经慢性压迫性损伤（CCI）大鼠的DRG中热敏性TRP通道的RNA水平。在经RTX处理的大鼠的DRG中，TRPV1和TRPA1的RNA显著降低，这表明TRPA1和TRPV1在感觉伤害感受器中存在功能共定位。在CCI大鼠的DRG中，TRPA1、TRPV2和TRPM8的RNA在周围神经损伤同侧显示出轻微但显著的增加。我们的研究结果支持以下假设：感觉神经元中TRP通道表达的增加可能导致机械性和冷觉超敏反应。
