Marquez D S, Ramírez L E, Moreno J, Pedrosa A L, Lages-Silva E
Departamento de Ciências Biológicas, Universidade Federal do Triângulo Mineiro, Avenida Frei Paulino, 30, Uberaba, Minas Gerais CEP 38025-180, Brazil.
Exp Parasitol. 2007 Sep;117(1):35-42. doi: 10.1016/j.exppara.2007.03.007. Epub 2007 Mar 24.
This study presents the first genetic characterization of five Trypanosoma rangeli isolates from Minas Gerais, in the southeast of Brazil and their comparison with Colombian populations by minicircle classification, RAPD-PCR and LSSP-PCR analyses. Our results demonstrated a homogenous T. rangeli population circulating among Didelphis albiventris as reservoir host in Brazil while heterogeneous populations were found in different regions of Colombia. KP1(+) minicircles were found in 100% isolates from Brazil and in 36.4% of the Colombian samples, whereas the KP2 and KP3 minicircles were detected in both groups. RAPD-PCR and LSSP-PCR profiles revealed a polymorphism within KP1(+) and KP1(-) T. rangeli populations and allowed the division of T. rangeli in two branches. The Brazilian KP1(+) isolates were more homogenous than the KP1(+) isolates from Colombia. The RAPD-PCR were entirely consistent with the distribution of KP1 minicircles while those obtained by LSSP-PCR were associated in 88.9% and 71.4% with KP1(+) and KP1(-) populations, respectively.
本研究首次对来自巴西东南部米纳斯吉拉斯州的5株朗氏锥虫分离株进行了基因特征分析，并通过微小环分类、随机扩增多态性PCR（RAPD-PCR）和长片段单链多态性PCR（LSSP-PCR）分析，将其与哥伦比亚种群进行比较。我们的结果表明，在巴西，朗氏锥虫在作为储存宿主的白腹袋鼬中传播的种群是同质的，而在哥伦比亚的不同地区发现了异质种群。在来自巴西的100%的分离株和36.4%的哥伦比亚样本中发现了KP1(+)微小环，而在两组中均检测到了KP2和KP3微小环。RAPD-PCR和LSSP-PCR图谱揭示了KP1(+)和KP1(-)朗氏锥虫种群内的多态性，并将朗氏锥虫分为两个分支。巴西的KP1(+)分离株比来自哥伦比亚的KP1(+)分离株更具同质性。RAPD-PCR结果与KP1微小环的分布完全一致，而LSSP-PCR结果分别与KP1(+)和KP1(-)种群的关联度为88.9%和71.4%。
