Ha Jung-Min, Kim Myoung-Rae, Oh Ho-Kyun, Lee Byung Hun, Ahn Hyun-Young, Shin Jong-Chul, Baek Sang Hong, Joe Young Ae
Cancer Research Institute and Department of Biomedical Science, College of Medicine, The Catholic University of Korea, Banpo-Dong 505, Seocho-Ku, Seoul, Republic of Korea.
FEBS Lett. 2007 Jun 12;581(14):2663-9. doi: 10.1016/j.febslet.2007.05.010. Epub 2007 May 15.
Outgrowing endothelial progenitor-derived cells (EPDCs) originate from a novel hierarchy of endothelial progenitor cells. In this study, EPDCs isolated from human cord blood were examined for phenotype and functional features upon aging. Young or aged EPDCs were similar to human umbilical vein endothelial cells (HUVECs), in exhibiting typical endothelial phenotypes. However, EPDCs were more sensitive to angiogenesis inducers or inhibitors in proliferation and migration. In addition, EPDCs underwent senescence markedly slowly with sustained endothelial NO synthase expression and activation, and their ability to undergo capillary morphogenesis was retained throughout longterm culture. Thus, these results suggest that a homogenous population of EPDCs derived from clonogenic expansion may provide an effective vasculogenesis tool.
生长成熟的内皮祖细胞（EPDCs）源自一种新型的内皮祖细胞层级结构。在本研究中，对从人脐带血中分离出的EPDCs进行了衰老过程中的表型和功能特征检测。年轻或衰老的EPDCs与人类脐静脉内皮细胞（HUVECs）相似，呈现典型的内皮表型。然而，EPDCs在增殖和迁移方面对血管生成诱导剂或抑制剂更为敏感。此外，EPDCs通过持续的内皮型一氧化氮合酶表达和激活，衰老明显缓慢，并且在长期培养过程中其形成毛细血管形态的能力得以保留。因此，这些结果表明，源自克隆性扩增的同质EPDC群体可能提供一种有效的血管生成工具。
