Duan Hua-Xin, Cheng La-Mei, Wang Jian, Hu Liang-Shan, Lu Guang-Xiu
National Center of Human Stem Cell Research and Engineering, Institute of Human Reproduction and Stem Cell Engineering, Central South University, 87 Xiang Ya Road, Changsha, Hunan 410078, China.
Cell Biol Int. 2006 Dec;30(12):1018-27. doi: 10.1016/j.cellbi.2006.08.002. Epub 2006 Aug 24.
The hierarchy of endothelial progenitor cells (EPCs) in human umbilical cord blood has been disclosed. In this study we compare, for the first time, the angiogenic potential difference between two types of EPCs. We cultured mononuclear cells (MNCs) isolated from human umbilical cord blood using endothelial cell-conditioned medium and obtained two types of EPCs, referred to as circulating angiogenic cells (CACs) and high proliferative potential endothelial progenitor cells (HPP-EPCs). Both types of cells possess characteristics of EPCs, including expressing CD31, VE-cadherin, KDR and von Willebrand factor, uptake of Ac-LDL and binding to lectin. However, unlike CACs, which express CD14 but not CD133, HPP-EPCs express CD133 but not CD14. Also, unlike CACs, HPP-EPCs display stronger proliferation and clonogenic potential in vitro and show stronger ability to promote vascular growth in the hind-limb model of ischemia in mice (BALB/C-nu) in vivo.
人类脐带血中内皮祖细胞（EPCs）的层级结构已被揭示。在本研究中，我们首次比较了两种类型EPCs之间的血管生成潜力差异。我们使用内皮细胞条件培养基培养从人类脐带血中分离出的单核细胞（MNCs），并获得了两种类型的EPCs，即循环血管生成细胞（CACs）和高增殖潜能内皮祖细胞（HPP-EPCs）。这两种类型的细胞都具有EPCs的特征，包括表达CD31、血管内皮钙黏蛋白、激酶插入域受体（KDR）和血管性血友病因子，摄取乙酰化低密度脂蛋白（Ac-LDL）以及与凝集素结合。然而，与表达CD14但不表达CD133的CACs不同，HPP-EPCs表达CD133但不表达CD14。此外，与CACs不同，HPP-EPCs在体外显示出更强的增殖和克隆形成潜力，并且在体内小鼠（BALB/C-nu）后肢缺血模型中表现出更强的促进血管生长的能力。
