Goossens Ellen, Frederickx Veerle, Geens Mieke, De Block Gert, Tournaye Herman
Center for Reproductive Medicine and Research Laboratories for Reproductive Medicine, University Hospital and Medical School, Dutch-Speaking Brussels Free University (Vrije Universiteit Brussel), Brussels, Belgium.
Fertil Steril. 2008 Mar;89(3):725-7. doi: 10.1016/j.fertnstert.2007.03.044. Epub 2007 May 22.
Although childhood cancer treatments are yielding higher survival rates, sterility remains one of the major side effects. For prepubertal boys there are currently no options to preserve fertility. Testicular tissue banking together with subsequent grafting may become a possible strategy in the future. In the present study, we compared two cryopreservation protocols using prepubertal murine testicular tissue. Fresh and cryopreserved testicular tissue was grafted subcutaneously on the back of immune-deficient mice for at least 3 months. Prepubertal murine tissue recovered well after cryopreservation with both ethylene glycol (EG) and dimethylsulfoxide (DMSO). While in fresh murine allografts, spermatozoa were observed in 23% of the tubules; in both the DMSO and the EG groups, 32% of the seminiferous tubules contained spermatozoa. However, with DMSO the structure of the seminiferous tubules was better preserved.
尽管儿童癌症治疗的生存率在不断提高，但不育仍然是主要的副作用之一。对于青春期前的男孩，目前尚无保留生育能力的选择。睾丸组织库及随后的移植可能会成为未来一种可行的策略。在本研究中，我们比较了两种使用青春期前小鼠睾丸组织的冷冻保存方案。将新鲜和冷冻保存的睾丸组织皮下移植到免疫缺陷小鼠的背部至少3个月。青春期前小鼠组织在使用乙二醇（EG）和二甲基亚砜（DMSO）冷冻保存后恢复良好。在新鲜的小鼠同种异体移植中，23%的小管中观察到精子；在DMSO组和EG组中，32%的生精小管含有精子。然而，使用DMSO时，生精小管的结构保存得更好。
