Shelepov V P, Chekulaev V A, Pasha-Zade G R
Laboratory of Tumour Biochemistry, USSR Cancer Research Centre, Academy of Medical Sciences, Moscow.
Biomed Sci. 1991;2(3):239-48.
The interrelationship between glycogen biosynthesis and do novo glucose formation in rats was studied by determining the oxaloacetic acid, ATP, and glycogen levels in the tissues of healthy starved rats and during the growth of transplantable hepatomas with different rates of growth. From the results obtained it was proposed that glycogen biosynthesis and de novo glucose formation are mutually enhanced and coupled processes. The validity of this conclusion was confirmed by in vitro model experiments involving the use of isolated-liver perfusion. Results of a multiple regression analysis indicated that the efficiency of gluconeogenesis and glycogen deposition is determined by the supply of oxygen to the liver, by the oxaloacetic acid content of this organ, and also by the degree of reduction of the glucose precursor. It was also shown that the ability of glucocorticoids and glucagon to enhance gluconeogenesis is dependent on the supply of oxygen to the liver. From the results of this in vivo and in vitro comparative study it is concluded that deposition of glycogen in the liver and possibly also in the brain is determined by the efficiency of gluconeogenesis.
通过测定健康饥饿大鼠组织以及不同生长速率的可移植肝癌生长过程中的草酰乙酸、三磷酸腺苷（ATP）和糖原水平，研究了大鼠糖原生物合成与从头合成葡萄糖之间的相互关系。根据所得结果，有人提出糖原生物合成与从头合成葡萄糖是相互促进且相互关联的过程。涉及使用离体肝脏灌注的体外模型实验证实了这一结论的有效性。多元回归分析结果表明，糖异生和糖原沉积的效率取决于肝脏的氧气供应、该器官的草酰乙酸含量以及葡萄糖前体的还原程度。还表明，糖皮质激素和胰高血糖素增强糖异生的能力取决于肝脏的氧气供应。从这项体内和体外比较研究的结果可以得出结论，肝脏以及可能还有大脑中的糖原沉积取决于糖异生的效率。
