Zhang Ying, Du Juan, Zhang Yong, Sun Hong-li, Pan Zhen-wei, Lu Yan-jie, Li Bao-xin, Yang Bao-feng
Department of Pharmacology, Bio-pharmaceutical Key Laboratory of Heilongjiang Province-Incubator of State Key Laboratory, Harbin Medical University, Harbin 150086, China.
Yao Xue Xue Bao. 2007 Feb;42(2):139-44.
Because HERG potassium channel has important effects on both proarrhythmia and antiarrhythmia, we use immunofluorescence and Western blotting methods to detect the expression of HERG channel of HERG-HEK cells in different concentrations of matrine, oxymatrine and resveratrol. The findings showed that both matrine (1 micromol x L(-1) ) and oxymatrine ( 1micromol x L (-1) ) increased HERG channel expression ( n = 5, P < 0. 05 ) , while matrine (100 micromol x L(-1) ) decreased HERG channel expression ( n = 5, P < 0. 05), resveratrol didn't affect HERG channel expression. In conclusion, different concentrations of matrine and oxymatrine affect HERG channel expression, while there is no relationship between resveratrol and HERG channel expression. It provides a theoretical support for the safety and mechanism of anti-arrhythmic drugs.
由于HERG钾通道在致心律失常和抗心律失常方面均具有重要作用，我们采用免疫荧光和蛋白质印迹法检测不同浓度的苦参碱、氧化苦参碱和白藜芦醇作用下HERG-HEK细胞中HERG通道的表达情况。结果显示，苦参碱（1 μmol·L⁻¹）和氧化苦参碱（1 μmol·L⁻¹）均能增加HERG通道表达（n = 5，P < 0.05），而苦参碱（100 μmol·L⁻¹）则降低HERG通道表达（n = 5，P < 0.05），白藜芦醇对HERG通道表达无影响。综上所述，不同浓度的苦参碱和氧化苦参碱会影响HERG通道表达，而白藜芦醇与HERG通道表达无相关性。这为抗心律失常药物的安全性及作用机制提供了理论支持。
