Ma Guo-chang, Chen Shu-qing, Zhu Zhen-hong, Huang Yan-shan, Pan Hui-qiang, Zhou Jin-bao
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310031, China.
Yao Xue Xue Bao. 2007 Feb;42(2):197-200.
Double antibody sandwich-type ELISA was used to detect rhG-CSF in serum to study the pharmacokinetics of rhG-CSF, PEG-rhG-CSF and rHSA-hG-CSF in mice and to confirm that PEGlyation and albumin fusion of rhG-CSF technology can prolong half-life of G-CSF. Pharmacokinetic parameters were calculated with 3P87 software. T1/2 s of rhG-CSF, PEG-rhG-CSF and rHSA-hG-CSF are 2. 1 , 14.2 and 10. 6 h, respectively. T1/2 s of PEG- rhG-CSF and rHSA-hG-CSF are 7, 5 times than T1/2 s of rhG-CSF, respectively. Tpeak s of PEG-rhG-CSF and rHSA-hG-CSF are 15, 13 times than Tpeak of rhG-CSF, respectively. The result of ELISA indicates that PEGlyation and albumin fusion of rhG-CSF technology can prolong half-life of G-CSF.
采用双抗体夹心型ELISA法检测血清中的重组人粒细胞集落刺激因子（rhG-CSF），以研究rhG-CSF、聚乙二醇化重组人粒细胞集落刺激因子（PEG-rhG-CSF）和重组人血清白蛋白-人粒细胞集落刺激因子融合蛋白（rHSA-hG-CSF）在小鼠体内的药代动力学，并证实rhG-CSF的聚乙二醇化和白蛋白融合技术可延长G-CSF的半衰期。药代动力学参数用3P87软件计算。rhG-CSF、PEG-rhG-CSF和rHSA-hG-CSF的半衰期（T1/2）分别为2.1、14.2和10.6小时。PEG-rhG-CSF和rHSA-hG-CSF的T1/2分别是rhG-CSF的T1/2的7.5倍。PEG-rhG-CSF和rHSA-hG-CSF的达峰时间（Tpeak）分别是rhG-CSF的Tpeak的15倍和13倍。ELISA结果表明，rhG-CSF的聚乙二醇化和白蛋白融合技术可延长G-CSF的半衰期。
