Nyiredy Szabolcs, Szucs Zoltán, Szepesy László
Gyógynövény Kutató Intézet Zrt., Budakalász, Lupaszigeti út 4.-2011.
Acta Pharm Hung. 2007;77(1):65-71.
A new procedure (SOS-LC) is described for the optimization of the HPLC stationary phase, using serially connected columns and the principle of the "PRISMA" model. In the course of the base measurements the retention factors (k) of the analytes were determined on three different base stationary phases; other parameters were fixed. By use of these data the k values were predicted in the cases of all theoretically combined stationary phases. These predictions resulted in numerous intermediate theoretical separations but only the optimal one was compiled and tested applying other parameters fixed as in the case of the base measurements. The overall selectivity of this separation was better than that of any individual stationary phase. SOS-LC is independent of the mechanism and the scale of separation and will be very advantageous in the case of hyphenated techniques (LC-MS and LC-NMR) due to stable mobile phase background.
本文描述了一种用于优化高效液相色谱（HPLC）固定相的新方法（SOS-LC），该方法使用串联柱以及“PRISMA”模型的原理。在基础测量过程中，在三种不同的基础固定相上测定了分析物的保留因子（k）；其他参数保持固定。利用这些数据，预测了所有理论上组合的固定相情况下的k值。这些预测产生了许多中间理论分离结果，但仅汇编并测试了最优的分离结果，测试时应用了与基础测量相同的固定其他参数。这种分离的整体选择性优于任何单个固定相。SOS-LC与分离机制和规模无关，并且由于流动相背景稳定，在联用技术（LC-MS和LC-NMR）中具有很大优势。
