Matsumura Goki, Ishihara Yoko, Miyagawa-Tomita Sachiko, Ikada Yoshito, Matsuda Shojiro, Kurosawa Hiromi, Shin'oka Toshiharu
Cardiovascular Surgery, Heart Institute of Japan, Tokyo Women's Medical University, Tokyo, Japan.
Tissue Eng. 2006 Nov;12(11):3075-83. doi: 10.1089/ten.2006.12.3075.
This study evaluated the endothelial function and mechanical properties of tissue-engineered vascular autografts (TEVAs) constructed with autologous mononuclear bone marrow cells (MN-BMCs) and a biodegradable scaffold using a canine inferior vena cava (IVC) model. MN-BMCs were obtained from a dog and seeded onto a biodegradable tubular scaffold consisting of polyglycolide fiber and poly(L-lactide-co-epsilon-caprolactone) sponge. This scaffold was implanted in the IVC of the same dog on the day of surgery. TEVAs were analyzed biochemically, biomechanically, and histologically after implantation. When TEVAs were explanted and stimulated with acetylcholine at 1 month, they produced nitrates and nitrites dose dependently. N(G)-nitro-L-arginine methylester significantly inhibited these reactions. With stimulation by acetylcholine, factor VIII-positive cells of TEVAs produced endothelial nitric oxide synthase proteins, and the ratio of endothelial nitric oxide synthase/s17 mRNA was similar among native IVC and TEVAs 1 and 3 months after implantation. TEVAs had biochemical properties and wall thickness similar to those of native IVC at 6 months after implantation, and tolerated venous pressure well without any problems such as calcification. The number of inflammatory cells in TEVAs and the ratio of CD4/s17 mRNA decreased significantly with time. These results indicate that TEVAs are a biocompatible material with functional endothelial cells and biomechanical properties and do not have unwanted side effects.
本研究使用犬下腔静脉（IVC）模型，评估了由自体单核骨髓细胞（MN - BMCs）和可生物降解支架构建的组织工程血管自体移植物（TEVAs）的内皮功能和力学性能。MN - BMCs取自一只狗，并接种到由聚乙交酯纤维和聚（L - 丙交酯 - 共 - ε - 己内酯）海绵组成的可生物降解管状支架上。该支架在手术当天植入同一只狗的IVC中。植入后对TEVAs进行生化、生物力学和组织学分析。当TEVAs在1个月时取出并用乙酰胆碱刺激时，它们剂量依赖性地产生硝酸盐和亚硝酸盐。N（G）-硝基 - L - 精氨酸甲酯显著抑制这些反应。在乙酰胆碱刺激下，TEVAs的VIII因子阳性细胞产生内皮型一氧化氮合酶蛋白，并且植入后1个月和3个月时，TEVAs的内皮型一氧化氮合酶/s17 mRNA比例与天然IVC相似。植入后6个月时，TEVAs具有与天然IVC相似的生化特性和壁厚，并且能很好地耐受静脉压力，没有钙化等任何问题。TEVAs中炎症细胞的数量以及CD4/s17 mRNA的比例随时间显著降低。这些结果表明，TEVAs是一种具有功能性内皮细胞和生物力学性能的生物相容性材料，且没有不良副作用。
