Ma Bao Feng, Liu Xiao Mei, Zhang Ai Xia, Wang Peng, Zhang Xiu Ming, Li Shu Nong, Lahn Bruce T, Xiang Andy Peng
Center for Stem Cell Biology and Tissue Engineering, Sun Yat-sen University, Guangzhou 510080, China.
Rejuvenation Res. 2007 Jun;10(2):205-14. doi: 10.1089/rej.2006.0532.
Neurospheres are free-floating heterogeneous spheroid structures maintained in culture and have been used for in vitro expansion of neural stem/progenitor cells. But the growth characteristics and proliferation kinetics of cell population in neurospheres are poorly understood. Using clonogenic culture and immunocytochemistry, we observed the growth dynamics of cell populations in neurospheres and identified key parameters relating to the growth dynamics of cells in neurospheres, including the fraction of dividing cells, cell cycle duration, time delay of first division, and survival rate of cells. Based on these parameters, we established two mathematical models that describe kinetic features of neural stem/progenitor cells under clonogenic conditions. These models provide a powerful tool to explain and predict the experimental outcome and clarify the cell growth characteristics of neural stem/progenitor cells.
神经球是在培养中维持的自由漂浮的异质球体结构，已被用于神经干/祖细胞的体外扩增。但是，人们对神经球中细胞群体的生长特性和增殖动力学了解甚少。我们采用克隆形成培养和免疫细胞化学方法，观察了神经球中细胞群体的生长动态，并确定了与神经球中细胞生长动态相关的关键参数，包括分裂细胞比例、细胞周期持续时间、首次分裂的时间延迟以及细胞存活率。基于这些参数，我们建立了两个数学模型，描述克隆形成条件下神经干/祖细胞的动力学特征。这些模型为解释和预测实验结果以及阐明神经干/祖细胞的细胞生长特性提供了有力工具。
