Komatsu Nahoko, Saijoh Kiyofumi, Kuk Cynthia, Liu Amber C, Khan Saba, Shirasaki Fumiaki, Takehara Kazuhiko, Diamandis Eleftherios P
Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, ON, Canada.
Exp Dermatol. 2007 Jun;16(6):513-9. doi: 10.1111/j.1600-0625.2007.00562.x.
Human tissue kallikreins are a family of 15 trypsin- or chymotrypsin-like secreted serine proteases (KLK1-KLK15). Many KLKs have been identified in normal stratum corneum (SC) and sweat, and are candidate desquamation-related proteases. We report quantification by enzyme-linked immunosorbent assay (ELISA) of KLK5, KLK6, KLK7, KLK8, KLK10, KLK11, KLK13 and KLK14 in the SC and serum of atopic dermatitis (AD) patients by ELISA, and examine their variation with clinical phenotype, correlation with blood levels of eosinophils, lactate dehydrogenase (LDH) and immunoglobulin E. The overall SC serine protease activities were also measured. In the SC of AD, all KLKs, except KLK11, were significantly elevated. The elevation of chymotrypsin-like KLK7 was predominant, compared with trypsin-like KLKs. The SC overall plasmin- and furin-like activities were significantly elevated, while trypsin- and chymotrypsin-like activities did not differ significantly. In the serum of AD patients, KLK8 was significantly elevated and KLK5 and KLK11 were significantly decreased. However, their serum levels were not modified by corticosteroid topical agents. The alterations of KLK levels in the SC of AD were more pronounced than those in the serum. KLK7 in the serum was significantly correlated with eosinophil counts in the blood of AD patients, while KLK5, KLK8 and KLK11 were significantly correlated with LDH in the serum. In conclusion, we report abnormal kallikrein levels in the SC and the serum of AD patients. KLKs might be involved in skin manifestation and/or focal/systemic inflammatory reactions in AD. Our data may contribute to a better understanding of the pathogenesis of AD.
人组织激肽释放酶是一个由15种胰蛋白酶或类胰凝乳蛋白酶样分泌型丝氨酸蛋白酶组成的家族（KLK1 - KLK15）。许多KLK已在正常角质层（SC）和汗液中被鉴定出来，并且是与脱屑相关的候选蛋白酶。我们通过酶联免疫吸附测定（ELISA）报告了特应性皮炎（AD）患者SC和血清中KLK5、KLK6、KLK7、KLK8、KLK10、KLK11、KLK13和KLK14的定量情况，并研究了它们随临床表型的变化、与血液中嗜酸性粒细胞、乳酸脱氢酶（LDH）和免疫球蛋白E水平的相关性。还测量了SC中总的丝氨酸蛋白酶活性。在AD的SC中，除KLK11外，所有KLK均显著升高。与类胰蛋白酶样KLK相比，类胰凝乳蛋白酶样KLK7的升高更为显著。SC中总的纤溶酶样和弗林蛋白酶样活性显著升高，而类胰蛋白酶样和类胰凝乳蛋白酶样活性无显著差异。在AD患者的血清中，KLK8显著升高，而KLK5和KLK11显著降低。然而，它们的血清水平不受局部皮质类固醇药物的影响。AD患者SC中KLK水平的改变比血清中更为明显。血清中的KLK7与AD患者血液中的嗜酸性粒细胞计数显著相关，而KLK5、KLK8和KLK11与血清中的LDH显著相关。总之，我们报告了AD患者SC和血清中激肽释放酶水平异常。KLK可能参与了AD的皮肤表现和/或局部/全身炎症反应。我们的数据可能有助于更好地理解AD的发病机制。
