Wolf Ingrid H, Kodama Kazuo, Cerroni Lorenzo, Kerl Helmut
Department of Dermatology, Medical University of Graz, Graz, Austria.
Am J Dermatopathol. 2007 Jun;29(3):237-41. doi: 10.1097/01.dad.0000211531.33670.94.
Topical imiquimod (IQ) is an effective treatment for genital warts and various malignant tumors of the skin. IQ acts through the Toll-like receptor 7 leading to the production of cytokines and chemokines such as interferons, interleukins, and growth factors. We investigated the composition of the inflammatory cell infiltrate before, during, and after the treatment of 10 superficial cutaneous malignancies (melanoma in situ (n = 4), melanoma metastasis (n = 1), squamous cell carcinoma in situ (n = 4), and basal cell carcinoma (n = 1) with 5% IQ cream. Immunophenotyping revealed in all cases during treatment an increased population of T-lymphocytes positive for CD3, CD4 and CD8, as well as a considerable number of cytotoxic cells (TIA-1+, granzyme B+) and plasmacytoid dendritic cells (CD 123+). These findings further support previous investigations that the antitumor effects of IQ result from an enhanced cytotoxic T-cell mediated immune response and from the recruitment of plasmacytoid dendritic cells to the skin. The population of infiltrative inflammatory cells was similar in all patients irrespective of the type of tumor.
局部应用咪喹莫特（IQ）是治疗尖锐湿疣和各种皮肤恶性肿瘤的有效方法。IQ通过Toll样受体7发挥作用，导致细胞因子和趋化因子的产生，如干扰素、白细胞介素和生长因子。我们研究了10例浅表皮肤恶性肿瘤（原位黑色素瘤（n = 4）、黑色素瘤转移灶（n = 1）、原位鳞状细胞癌（n = 4）和基底细胞癌（n = 1））在使用5% IQ乳膏治疗前、治疗期间和治疗后的炎性细胞浸润成分。免疫表型分析显示，在治疗期间的所有病例中，CD3、CD4和CD8阳性的T淋巴细胞数量增加，以及相当数量的细胞毒性细胞（TIA-1+、颗粒酶B+）和浆细胞样树突状细胞（CD123+）。这些发现进一步支持了先前的研究，即IQ的抗肿瘤作用源于细胞毒性T细胞介导的免疫反应增强以及浆细胞样树突状细胞向皮肤的募集。所有患者的浸润性炎性细胞群体相似，与肿瘤类型无关。
