Sanchez-Bernal C, Vlitos M, Cabezas J A, Price R G
Departamento de Bioquimica, Facultad de Biologia, Universidad de Salamanca, Spain.
Cell Biochem Funct. 1991 Jul;9(3):209-14. doi: 10.1002/cbf.290090309.
Three aminoglycosidic antibiotics: tobramycin, amikacin and sisomicin were administered to rats. There was an increase in the activity of N-acetyl-beta-D-glucosaminidase (NAG) excreted in the urine and this was characterized by a change in the isoenzyme profiles eluted from DEAE--cellulose. The largest increase in NAG activity was observed following sisomicin administration due mainly to an increase in the B-form of NAG with a concomitant fall in the intermediate (I-form). Separation of urinary proteins by SDS-polyacrylamide gel electrophoresis demonstrated a mixed tubular and glomerular proteinuria following administration of sisomicin. It is concluded that the separation of NAG isoenzymes and urinary proteins provides valuable additional information on the nature and severity of antibiotic nephrotoxicity.
妥布霉素、阿米卡星和西索米星。尿中排泄的N - 乙酰 - β - D - 氨基葡萄糖苷酶（NAG）活性增加，其特征在于从DEAE - 纤维素洗脱的同工酶谱发生变化。西索米星给药后观察到NAG活性增加最大，这主要是由于NAG的B型增加，同时中间型（I型）下降。通过SDS - 聚丙烯酰胺凝胶电泳分离尿蛋白显示，西索米星给药后出现混合性肾小管和肾小球蛋白尿。结论是，NAG同工酶和尿蛋白的分离为抗生素肾毒性的性质和严重程度提供了有价值的额外信息。
