Bonifaz Alexandro, Isa-Isa Rafael, Araiza Javier, Cruz Cecilia, Hernández Marco A, Ponce Rosa Maria
Dermatology Service & Mycology Department, Hospital General de México OD, Sanchez Azcona 317 int 202, Col del Valle, Mexico, DF, Mexico.
Mycopathologia. 2007 Jun;163(6):309-13. doi: 10.1007/s11046-007-9019-6. Epub 2007 May 23.
This is a comparative study to isolate the dermatophytes of tinea capitis using the cytobrush and comparing it versus the standard method. A prospective, observational, comparative trial of 178 probable cases of tinea capitis was conducted in two dermatological centers. Each patient underwent mycological tests that included direct exam with KOH and cultures with either of two methods: scraping the scalp to remove hair and cell debris, and the cytobrush. A total of 135 clinically and mycologically proven cases of tinea capitis were included; 119 were non-inflammatory and 16 inflammatory tinea. A total of 131 had a positive direct exam and subsequent primary isolation cultures were obtained in 135 cases. The main dermatophytes isolated were Microsporum canis (68%) and Trichophyton tonsurans (20%). A total of 115/135 (85.1%), were detected with the traditional method, with an average of 11.2 days until positive, while the number detected with the cytobrush was 132/135 (97.7%) with an average of 8.5 days until positive. The chi-square statistical method showed that the cytobrush culture was superior to the standard one with a chi-square of 5.078 (P = 0.025), with a statistically significant difference versus the standard method.
这是一项比较研究，旨在使用细胞刷分离头癣的皮肤癣菌，并将其与标准方法进行比较。在两个皮肤科中心对178例疑似头癣病例进行了一项前瞻性、观察性比较试验。每位患者都接受了真菌学检测，包括KOH直接检查以及两种方法之一的培养：刮取头皮以去除毛发和细胞碎片，以及使用细胞刷。总共纳入了135例临床和真菌学确诊的头癣病例；其中119例为非炎症性头癣，16例为炎症性头癣。总共131例直接检查呈阳性，135例均获得了后续的初次分离培养。分离出的主要皮肤癣菌为犬小孢子菌（68%）和断发毛癣菌（20%）。传统方法检测出115/135（85.1%），平均11.2天呈阳性，而细胞刷检测出132/135（97.7%），平均8.5天呈阳性。卡方统计方法显示，细胞刷培养优于标准方法，卡方值为5.078（P = 0.025），与标准方法相比具有统计学显著差异。
