[白芍的高效液相色谱指纹图谱]

[HPLC fingerprinting of radix paeoniae alba].

作者信息

Yang Liu, Xu Shun-Jun, Tian Run-Tao, Xie Pei-Shan, Wang Zheng-Tao

机构信息

Second Affiliated Hospital, Guangzhou University of TCM, Guangzhou 510120, China.

出版信息

Yao Xue Xue Bao. 2007 Jan;42(1):71-4.

PMID:17520811

Abstract

To establish a sensitive and specific HPLC method for quality control of Radix Paeoniae Alba, HPLC method was applied for quality assessment of Radix Paeoniae Alba. HPLC analysis was performed on a Symmetry C18 column (250 mm x 4. 6 mm ID, 5 microm, Waters, USA). The mobile phase consisted of acetonitrile (solvent A) and water containing 0.1% (v/v) phosphoric acid (solvent B) at a constant flow rate of 0.8 mL x min(-1). An increasing linear gradient (v/v) of solvent A was used (t/min, % A): (0,10), (5,10), (25,15), (45, 22), (46, 65), (50, 80) and (60, 80). The column temperature was set at 25 degrees C. The chromatograms were monitored at 230 nm and the on-line UV spectra were recorded in the range of 190 - 400 nm. The HPLC chromatographic fingerprinting of Radix Paeoniae Alba, showing 11 characteristic peaks, was established from 28 lots of Radix Paeoniae Alba. The areas of main chromatographic peaks were found to complied with the following rule: paeoniflorin > 1, 2, 3, 4, 6-penta-O-galloyl-glucos > albiflorin > methyl gallate > other compounds. The chromatographic fingerprinting of Radix Paeoniae Alba with high specificity can be used to control its quality and assure lot-to-lot consistency.

摘要

为建立一种灵敏且特异的用于白芍质量控制的高效液相色谱（HPLC）方法，采用HPLC法对白芍进行质量评价。HPLC分析在Symmetry C18柱（250 mm×4.6 mm内径，5μm，美国沃特世公司）上进行。流动相由乙腈（溶剂A）和含0.1%（v/v）磷酸的水（溶剂B）组成，流速恒定为0.8 mL·min⁻¹。采用溶剂A的线性梯度增加（v/v）（t/min，% A）：（0，10），（5，10），（25，15），（45，22），（46，65），（50，80）和（60，80）。柱温设定为25℃。色谱图在230 nm处监测，在线紫外光谱在190 - 400 nm范围内记录。从28批白芍中建立了显示11个特征峰的白芍HPLC色谱指纹图谱。发现主要色谱峰面积符合以下规律：芍药苷＞1,2,3,4,6 - 五没食子酰葡萄糖＞ albiflorin＞没食子酸甲酯＞其他化合物。具有高特异性的白芍色谱指纹图谱可用于控制其质量并确保批次间的一致性。