高效液相色谱法测定双丹颗粒中丹酚酸B和芍药苷的含量
[Determination of salvianolic acid B and paeoniflorin in Shuangdan granules by HPLC].
作者信息
Ge Zhi-wei, He Qing, Shui Wen-bo, Cheng Yi-yu
机构信息
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2006 Jul;31(13):1062-4.
OBJECTIVE
To develop a method for the determination of salvianolic acid B and paeoniflorin in Shuangdan granules.
METHOD
The chromatographic separation was performed on a Zorbax SB C18 column with a linear gradient elution of phosphoric acid-water (0.05:100) and phosphoric acid-acetonitrile-methanol (0.05:4:96) was used. The flow rate was 1 mL x min(-1) and column temperature was set at 25 degrees C. The UV detection wavelength was set at 230 nm.
RESULT
The linear ranger was of 0.710-22.7 microg for salvianolic acid B and 0.103-3.30 microg for paeoniflorin. Three batches of Shuangdan granules were analyzed, and the content of salvianolic acid B ranged from 6.46-11.6 mg x g(-1) and paeoniflorin ranged from 1.44-1.78 mg x g(-1).
CONCLUSION
The method was accurate, reproducible, reliable, and could be used for quantitative control of Shuangdan granules.
目的
建立双丹颗粒中丹酚酸B和芍药苷的含量测定方法。
方法
采用Zorbax SB C18柱进行色谱分离，以磷酸-水（0.05:100）和磷酸-乙腈-甲醇（0.05:4:96）进行线性梯度洗脱。流速为1 mL·min⁻¹，柱温设定为25℃，紫外检测波长设定为230 nm。
结果
丹酚酸B的线性范围为0.710 - 22.7 μg，芍药苷的线性范围为0.103 - 3.30 μg。对三批双丹颗粒进行分析，丹酚酸B的含量范围为6.46 - 11.6 mg·g⁻¹，芍药苷的含量范围为1.44 - 1.78 mg·g⁻¹。
结论
该方法准确、重现性好、可靠，可用于双丹颗粒的定量控制。
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