Tsvetkov T, Takeva T
Institute for Cryobiology and Lyophilization, Sofia, Bulgaria.
Cryobiology. 1991 Oct;28(5):503-8. doi: 10.1016/0011-2240(91)90061-r.
The ovaries of 36 ground squirrels were studied in March, April, July, and December. Morphological and functional seasonal characteristics of theca interna were studied by histological methods, electron microscopy, and quantitative methods for determinating the volume density and histoenzyme activity of NADH2-tetrazolium reductase, glucose-6-phosphate dehydrogenase, and delta 5-3 beta-hydroxysteroid dehydrogenase. Maximal activity of theca interna as a steroid-producing structure was observed during spring awakening. A significant increase in the histoenzyme activity of delta 5-3 beta-hydroxysteroid dehydrogenase in December, versus two other enzymes studied, indicated steroid synthesis in theca terna.
在三月、四月、七月和十二月对36只地松鼠的卵巢进行了研究。采用组织学方法、电子显微镜以及测定NADH2 - 四氮唑还原酶、葡萄糖 - 6 - 磷酸脱氢酶和δ5 - 3β - 羟基类固醇脱氢酶的体积密度和组织酶活性的定量方法，研究了卵泡内膜的形态和功能季节性特征。作为类固醇生成结构的卵泡内膜的最大活性在春季苏醒期间被观察到。与所研究的其他两种酶相比，十二月时δ5 - 3β - 羟基类固醇脱氢酶的组织酶活性显著增加，表明卵泡膜中存在类固醇合成。
