Hawley H P, Gordon G B
Lab Invest. 1976 Feb;34(2):216-22.
Neutrophils from healthy volunteers were isolated and incubated with varying concentrations of albumin-bound long chain free fatty acids. Standard in vitro function tests including phagocytosis, bactericidal activity, and chemotaxis were performed after the incubation. It was found that unsaturated fatty acid (oleic acid) caused no changes in bactericidal activity and only moderate decreases in phagocytosis and chemotaxis at very high concentrations. Saturated fatty acid (palmitic acid) produced, at high concentrations, virtually complete inhibition of chemotaxis and moderate depression of phagocytosis and bactericidal ability. Most significantly, lower concentrations of saturated free fatty acids, within the range reported clinically in various diseases, caused a marked inhibition of chemotaxis. These functional disturbances were associated with ultrastructural alterations. Neutrophils treated with oleic acid contained numerous cytoplasmic neutral lipid droplets. Neutrophils incubated with palmitic acid showed elongated cleftlike dilations of the endoplasmic reticulum and degenerative degranulated cytoplasmic areas. It is postulated that these represent crystallization of excess saturated free fatty acids or triglyceride which interfere with chemotaxis, either mechanically or by causing cell injury.
从健康志愿者体内分离出中性粒细胞，并与不同浓度的白蛋白结合长链游离脂肪酸一起孵育。孵育后进行包括吞噬作用、杀菌活性和趋化性在内的标准体外功能测试。结果发现，不饱和脂肪酸（油酸）对杀菌活性无影响，仅在极高浓度下吞噬作用和趋化性有适度降低。饱和脂肪酸（棕榈酸）在高浓度时几乎完全抑制趋化性，对吞噬作用和杀菌能力有中度抑制。最显著的是，在各种疾病临床报道的浓度范围内，较低浓度的饱和游离脂肪酸会显著抑制趋化性。这些功能紊乱与超微结构改变有关。用油酸处理的中性粒细胞含有大量细胞质中性脂滴。与棕榈酸孵育的中性粒细胞显示内质网呈细长的裂隙状扩张，细胞质区域出现退行性脱颗粒。据推测，这些代表了过量饱和游离脂肪酸或甘油三酯的结晶，它们通过机械作用或导致细胞损伤来干扰趋化性。
