Suppr超能文献

游离脂肪酸对淋巴细胞增殖的抑制作用。II. 硬脂酸对植物血凝素激活的T细胞的毒性。

Inhibition of lymphocyte proliferation by free fatty acids. II. Toxicity of stearic acid towards phytohaemagglutinin-activated T cells.

作者信息

Buttke T M, Cuchens M A

出版信息

Immunology. 1984 Nov;53(3):507-14.

PMID:6333387
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1454918/
Abstract

Studies were performed to further characterize the effects of saturated fatty acids on murine T lymphocyte proliferation. Flow cytometry was used to show that the inhibitory effects of stearic acid (18:0) on [3H]thymidine uptake can be correlated with changes in cellular DNA content. Additional studies using flow cytometry and fluorescein diacetate as a viability stain showed that exogenous 18:0 was toxic for phytohemagglutinin (PHA)-stimulated T cells, whereas the viability of unstimulated T cells was less affected by 18:0. The inhibitory effects of 18:0 on T cell proliferation were evident as early as 4 hr after fatty acid addition and after a 10-hr exposure, the effects of 18:0 could not be reversed by washing the cells or by adding oleic acid (18:1). It is proposed that the inhibitory effects of 18:0 are dependent upon PHA-induced changes in T cell lipid metabolism.

摘要

开展了多项研究以进一步明确饱和脂肪酸对小鼠T淋巴细胞增殖的影响。采用流式细胞术显示，硬脂酸（18:0）对[3H]胸腺嘧啶核苷摄取的抑制作用与细胞DNA含量的变化相关。使用流式细胞术和荧光素二乙酸酯作为活力染色剂的其他研究表明，外源性18:0对植物血凝素（PHA）刺激的T细胞有毒性，而未刺激的T细胞活力受18:0的影响较小。早在添加脂肪酸后4小时，18:0对T细胞增殖的抑制作用就很明显，在暴露10小时后，通过洗涤细胞或添加油酸（18:1）无法逆转18:0的作用。有人提出，18:0的抑制作用取决于PHA诱导的T细胞脂质代谢变化。

相似文献

1
Inhibition of lymphocyte proliferation by free fatty acids. II. Toxicity of stearic acid towards phytohaemagglutinin-activated T cells.游离脂肪酸对淋巴细胞增殖的抑制作用。II. 硬脂酸对植物血凝素激活的T细胞的毒性。
Immunology. 1984 Nov;53(3):507-14.
2
Inhibition of lymphocyte proliferation by free fatty acids. III. Modulation of thymus-dependent immune responses.游离脂肪酸对淋巴细胞增殖的抑制作用。III. 胸腺依赖性免疫反应的调节
Immunology. 1985 Dec;56(4):659-66.
3
Inhibition of lymphocyte proliferation by free fatty acids. I. Differential effects on mouse B and T lymphocytes.游离脂肪酸对淋巴细胞增殖的抑制作用。I. 对小鼠B淋巴细胞和T淋巴细胞的不同影响。
Immunology. 1984 Oct;53(2):235-42.
4
Molecular basis for the immunosuppressive action of stearic acid on T cells.硬脂酸对T细胞免疫抑制作用的分子基础。
Immunology. 1990 Jul;70(3):379-84.
5
Functional heterogeneity in the process of T lymphocyte activation; barium blocks several modes of T cell activation, but spares a functionally unique subset of PHA-activable T cells.T淋巴细胞激活过程中的功能异质性；钡可阻断T细胞激活的多种模式，但不影响功能独特的可被PHA激活的T细胞亚群。
Clin Exp Immunol. 1989 May;76(2):311-6.
6
The effect of tri-iodothyronine on the phytohaemagglutinin response of T lymphocytes.三碘甲状腺原氨酸对T淋巴细胞植物血凝素反应的影响。
Clin Exp Immunol. 1982 Jan;47(1):217-20.
7
Effects of 5-mercapto-2'-deoxyuridine on the incorporation of nucleosides into RNA and DNA in a primary lymphocyte culture system.5-巯基-2'-脱氧尿苷对原代淋巴细胞培养系统中核苷掺入RNA和DNA的影响。
Cancer Res. 1976 Sep;36(9 pt.1):3284-93.
8
The biologic activity of the immunoregulatory lipoprotein, LDL-In is independent of its free fatty acid content.免疫调节脂蛋白LDL-In的生物活性与其游离脂肪酸含量无关。
J Immunol. 1981 Apr;126(4):1382-6.
9
[Effects of Hesperetin on cell activation and proliferation of murine T lymphocytes in vitro].橙皮素对小鼠T淋巴细胞体外细胞活化及增殖的影响
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2009 Apr;25(4):315-8.
10
Specific inhibitory action of polyunsaturated fatty acids on lymphocyte transformation induced by PHA and PPD.多不饱和脂肪酸对PHA和PPD诱导的淋巴细胞转化的特异性抑制作用。
Int Arch Allergy Appl Immunol. 1975;48(2):203-10. doi: 10.1159/000231306.

引用本文的文献

1
Lipid Metabolism and Immune Function: Chemical Tools for Insights into T-Cell Biology.脂质代谢与免疫功能：深入了解T细胞生物学的化学工具
Chembiochem. 2025 Jun 3;26(11):e202400980. doi: 10.1002/cbic.202400980. Epub 2025 Apr 16.
2
Prediction of acute GVHD and relapse by metabolic biomarkers after allogeneic hematopoietic stem cell transplantation.异基因造血干细胞移植后代谢生物标志物预测急性移植物抗宿主病和复发。
JCI Insight. 2018 May 3;3(9):99672. doi: 10.1172/jci.insight.99672.
3
Effect of Diet and Exercise on the Peripheral Immune System in Young Balb/c Mice.饮食和运动对年轻Balb/c小鼠外周免疫系统的影响。
Biomed Res Int. 2015;2015:458470. doi: 10.1155/2015/458470. Epub 2015 Nov 8.
4
Mycoplasma hyopneumoniae p65 surface lipoprotein is a lipolytic enzyme with a preference for shorter-chain fatty acids.猪肺炎支原体p65表面脂蛋白是一种优先作用于短链脂肪酸的脂解酶。
J Bacteriol. 2004 Sep;186(17):5790-8. doi: 10.1128/JB.186.17.5790-5798.2004.
5
Identification of Aeromonas hydrophila hybridization group 1 by PCR assays.通过聚合酶链反应（PCR）检测鉴定嗜水气单胞菌杂交组1
Appl Environ Microbiol. 1996 Apr;62(4):1167-70. doi: 10.1128/aem.62.4.1167-1170.1996.
6
Purification, gene cloning, amino acid sequence analysis, and expression of an extracellular lipase from an Aeromonas hydrophila human isolate.嗜水气单胞菌人源分离株胞外脂肪酶的纯化、基因克隆、氨基酸序列分析及表达
Appl Environ Microbiol. 1993 Aug;59(8):2411-7. doi: 10.1128/aem.59.8.2411-2417.1993.
7
A lipase of Aeromonas hydrophila showing nonhemolytic phospholipase C activity.嗜水气单胞菌的一种具有非溶血磷脂酶C活性的脂肪酶。
Curr Microbiol. 1995 Jul;31(1):28-33. doi: 10.1007/BF00294630.
8
Interference of Staphylococcus aureus lipase with human granulocyte function.金黄色葡萄球菌脂肪酶对人粒细胞功能的干扰。
Eur J Clin Microbiol Infect Dis. 1988 Aug;7(4):505-10. doi: 10.1007/BF01962601.
9
Inhibition of lymphocyte proliferation by free fatty acids. III. Modulation of thymus-dependent immune responses.游离脂肪酸对淋巴细胞增殖的抑制作用。III. 胸腺依赖性免疫反应的调节
Immunology. 1985 Dec;56(4):659-66.
10
Absence of unsaturated fatty acid synthesis in murine T lymphocytes.小鼠T淋巴细胞中不饱和脂肪酸合成的缺失。
Proc Natl Acad Sci U S A. 1989 Aug;86(16):6133-7. doi: 10.1073/pnas.86.16.6133.

本文引用的文献

1
Incubation of exogenous fatty acids with lymphocytes. Changes in fatty acid composition and effects on the rotational relaxation time of 1,6-diphenyl-1,3,5-hexatriene.外源性脂肪酸与淋巴细胞的孵育。脂肪酸组成的变化以及对1,6-二苯基-1,3,5-己三烯旋转弛豫时间的影响。
Biochemistry. 1980 Jun 10;19(12):2756-62. doi: 10.1021/bi00553a034.
2
The relationship between plasma membrane lipid composition and physical-chemical properties. I. Fluorescence polarization studies of fatty acid-altered EL4 tumor cell membranes.质膜脂质组成与物理化学性质之间的关系。I. 脂肪酸改变的EL4肿瘤细胞膜的荧光偏振研究。
Biochim Biophys Acta. 1981 Mar 20;642(1):106-18. doi: 10.1016/0005-2736(81)90142-5.
3
The effect of concanavalin A on the incorporation of fatty acids into lymphocyte phospholipids.伴刀豆球蛋白A对脂肪酸掺入淋巴细胞磷脂的影响。
J Pharmacobiodyn. 1983 Mar;6(3):217-24. doi: 10.1248/bpb1978.6.217.
4
Membrane structure-function relationships in cell-mediated cytolysis. I. Effect of exogenously incorporated fatty acids on effector cell function in cell-mediated cytolysis.细胞介导的细胞溶解中的膜结构 - 功能关系。I. 外源掺入脂肪酸对细胞介导的细胞溶解中效应细胞功能的影响。
J Immunol. 1980 Aug;125(2):689-95.
5
Influence of fatty acyl substitution on the composition and function of macrophage membranes.脂肪酰基取代对巨噬细胞膜组成和功能的影响。
J Biol Chem. 1980 May 25;255(10):4910-7.
6
Modulation of cell-mediated cytotoxicity function after alteration of fatty acid composition in vitro.体外脂肪酸组成改变后细胞介导的细胞毒性功能的调节
J Immunol. 1984 Jan;132(1):81-7.
7
Lyb-5- B cells of CBA/N mice can be induced to synthesize DNA by culture with insolubilized but not soluble anti-Ig.CBA/N小鼠的Lyb-5-B细胞通过与不溶性而非可溶性抗Ig培养可被诱导合成DNA。
J Immunol. 1983 Nov;131(5):2107-9.
8
Positive selection of mouse B and T lymphocytes and analysis of isolated populations by flow cytometry.小鼠B淋巴细胞和T淋巴细胞的阳性选择以及通过流式细胞术对分离群体的分析。
J Immunol Methods. 1983 Mar 11;58(1-2):193-207. doi: 10.1016/0022-1759(83)90275-2.
9
Modulation of enzyme activities in isolated lymphocyte plasma membranes by enzymatic modification of phospholipid fatty acids.通过磷脂脂肪酸的酶促修饰对分离淋巴细胞质膜中酶活性的调节。
J Biol Chem. 1981 Nov 25;256(22):11618-23.
10
Membrane properties of living mammalian cells as studied by enzymatic hydrolysis of fluorogenic esters.通过荧光酯的酶促水解研究活的哺乳动物细胞的膜特性。
Proc Natl Acad Sci U S A. 1966 Jan;55(1):134-41. doi: 10.1073/pnas.55.1.134.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验