对迁移路径进行间歇扫描的电泳：在更短时间内检测分辨率

Electrophoresis with intermittent scanning of the migration path: detection of resolution within shortened time.

作者信息

Deml M, Garner M M, Chrambach A

机构信息

Section on Macromolecular Analysis, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892.

出版信息

Electrophoresis. 1991 Sep;12(9):641-5. doi: 10.1002/elps.1150120908.

DOI:10.1002/elps.1150120908

PMID:1752244

Abstract

Intermittent optical scanning (by detection of optical density or fluorescence) of the electrophoretic migration path was applied to the resolution of two dyes under an arbitrary set of conditions. Scanning at 5-min intervals allows for detection of resolution between the two zones at least 3 times faster than conventional automatic zone detection employing a detector at the end of the migration path. This result promises that replacing stationary by mobile detectors in general would result in a substantial time saving for automated detection of electrophoretic zones.

摘要

通过对电泳迁移路径进行间歇性光学扫描（检测光密度或荧光），在任意设定条件下对两种染料进行了分离。每隔5分钟进行一次扫描，与在迁移路径末端使用检测器的传统自动区带检测相比，检测两个区带之间分辨率的速度至少快3倍。这一结果表明，一般而言，用移动检测器取代固定检测器将大幅节省电泳区带自动检测的时间。