Witzmann F, Jarnot B, Parker D
Molecular Anatomy Laboratory, Indiana University-Purdue University, Indianapolis, Columbus 47203.
Electrophoresis. 1991 Sep;12(9):687-8. doi: 10.1002/elps.1150120919.
This report describes the incorporation of an alkyl maltoside detergent in two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) sample lysis buffer in order to improve resolution of protein patterns separated by nonequilibrium pH gradient electrophoresis. Membrane-associated proteins with alkaline isoelectric points form horizontal streaks on two-dimensional electrophoretograms when solubilized with conventional nonionic detergent. Dodecyl maltoside enhances protein delipidation during solubilization and improves pattern resolution and protein mobility.
本报告描述了在二维聚丙烯酰胺凝胶电泳（2-D PAGE）样品裂解缓冲液中加入烷基麦芽糖苷去污剂，以提高非平衡pH梯度电泳分离的蛋白质图谱的分辨率。当用传统的非离子去污剂溶解时，具有碱性等电点的膜相关蛋白在二维电泳图上形成水平条纹。十二烷基麦芽糖苷在溶解过程中增强了蛋白质的脱脂作用，并提高了图谱分辨率和蛋白质迁移率。
