Salaj Terezia, Panis Bart, Swennen Rony, Salaj Jan
Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Nitra, Slovak Republic.
Cryo Letters. 2007 Mar-Apr;28(2):69-76.
Six different embryogenic cell lines of Pinus nigra Arn. have been cryopreserved in liquid nitrogen using cryoprotection with sucrose (18%) and DMSO (7.5%). Post-thaw growth and tissue proliferation have been observed in five cell lines. The survival levels after storage in liquid nitrogen reached values between 62.5 and 100%. Growth of recovered embryogenic cells as well as somatic embryos is similar to the non-frozen tissues maintained in long-term culture. Somatic embryo maturation and plantlet regeneration occurred in all selected cell lines.
欧洲黑松（Pinus nigra Arn.）的六种不同胚性细胞系已使用18%蔗糖和7.5%二甲基亚砜进行冷冻保护后保存在液氮中。解冻后，在五个细胞系中观察到了生长和组织增殖。液氮保存后的存活率达到了62.5%至100%。复苏的胚性细胞以及体细胞胚的生长与长期培养中保存的未冷冻组织相似。所有选定的细胞系中都发生了体细胞胚成熟和植株再生。
