利用cDNA微阵列研究人脐静脉内皮细胞（HUVECs）的热休克反应。
Study of heat shock response of human umbilical vein endothelial cells (HUVECs) using cDNA microarray.
作者信息
Zhou M, Zhang A, Lin B, Liu J, Xu L X
机构信息
Key Laboratory of Systems Biomedicine, Shanghai Jiao Tong University, Shanghai 200240, P. R. China.
出版信息
Int J Hyperthermia. 2007 May;23(3):225-58. doi: 10.1080/02656730701295441.
Genetic response of human umbilical vein cells (HUVECs) to heat shock was studied using gene expression analysis. HUVECs were subjected to heat treatment at 44 degrees C and 55 degrees C for 1 h, respectively. Four hours after the treatment, gene expression of the treated cells and control cells (37 degrees C) were profiled using cDNA microarray. Data were analyzed using bioinformatics tools, and the results were verified by real-time quantitative PCR experiment. It has been shown that a large number of genes were regulated indicating global response to heat treatment at the genetic level.
利用基因表达分析研究了人脐静脉细胞（HUVECs）对热休克的遗传反应。分别将HUVECs在44℃和55℃下进行1小时的热处理。处理后4小时，使用cDNA微阵列对处理过的细胞和对照细胞（37℃）的基因表达进行分析。使用生物信息学工具对数据进行分析，并通过实时定量PCR实验对结果进行验证。结果表明，大量基因受到调控，表明在遗传水平上对热处理有整体反应。
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