Sheng Xiu-zhen, Zhan Wen-bin, Wang Yue
Laboratory of Pathology and Immunology of Aquatic Animals, LMMEC, Ocean University of China, 5 Yushan Road, Qingdao 266003, PR China.
Dis Aquat Organ. 2007 Mar 29;75(1):23-8. doi: 10.3354/dao075023.
In April 2004 white nodular lesions were found on the fins of whitespotted puffer Arothron hispidus (Linnaeus). Diagnostic studies were carried out to confirm the disease using light and electron microscopy, histochemical methods and PCR. The results revealed that the nodules were composed of giant cells up to 400 microm in diameter. These cells were surrounded by a periodic acid-Schiff (PAS)-positive hyaline capsule containing dot-shaped, Feulgen-positive inclusion bodies in the cytoplasm and an irregular nucleus. Numerous virus particles 200 nm in diameter and with hexagonal profiles were observed in the cytoplasm. These features were consistent with those of lymphocystis disease. Additionally, based on the gene sequences of major capsid protein (MCP) of lymphocystis disease virus (LCDV) from Japanese flounder Paralichthys olivaceus, 2 pair primers were designed; after a nested PCR was performed for detection of LCDV in A. hispidus, a positive amplified product was obtained showing the presence of LCDV. Therefore, the white nodules were the lymphocystis lesions caused by LCDV infection and A. hispidus was demonstrated to be a new host for LCDV.
2004年4月，在白点叉鼻鲀（Arothron hispidus，林奈命名）的鱼鳍上发现了白色结节性病变。利用光学显微镜和电子显微镜、组织化学方法及聚合酶链反应（PCR）进行了诊断研究以确诊该病。结果显示，这些结节由直径达400微米的巨细胞组成。这些细胞被一个过碘酸希夫（PAS）阳性的透明囊包围，囊内细胞质中有点状、福尔根阳性的包涵体以及一个不规则的细胞核。在细胞质中观察到许多直径为200纳米且呈六边形轮廓的病毒颗粒。这些特征与淋巴囊肿病相符。此外，根据来自日本牙鲆（Paralichthys olivaceus）的淋巴囊肿病病毒（LCDV）主要衣壳蛋白（MCP）的基因序列设计了2对引物；对白点叉鼻鲀进行LCDV检测的巢式PCR后，获得了阳性扩增产物，表明存在LCDV。因此，这些白色结节是由LCDV感染引起的淋巴囊肿病变，且白点叉鼻鲀被证明是LCDV的新宿主。
