Matsuyama Tomomasa, Fujiwara Atushi, Nakayasu Chihaya, Kamaishi Takashi, Oseko Norihisa, Tsutsumi Nobuyuki, Hirono Ikuo, Aoki Takashi
National Research Institute of Aquaculture, Fisheries Research Agency, Minami-Ise, Mie 516-0193, Japan.
Dis Aquat Organ. 2007 Mar 29;75(1):79-83. doi: 10.3354/dao075079.
In this study, the gene expression patterns of peripheral blood leucocytes (PBL) from Japanese flounder Paralichthys olivaceus were analyzed during the course of monogenean parasite Neoheterobothrium hirame infection in order to select candidates for molecular biomarkers of infection. cDNA microarray analysis was performed to compare the gene expression patterns of PBL between infected and non-infected fishes. Among the 797 genes analyzed, 45 genes (5.6%) changed their expression levels. These genes included specific and non-specific immune-related genes (matrix metalloproteinase[MMP]-9, MMP-13, leukotriene B4 receptor, CD20 receptor, MHC [major histocompatibility complex] Class I, MHC Class II beta-chain, immunoglobulin light chain and immunoglobulin heavy chain). Significant up- and down-regulation of some unknown genes was also observed. Several candidates for infection-marker genes were selected for further study. These genes included MMP-9, MMP-13, leukotriene b4 receptor, CD20 receptor, immunoglobulin heavy chain, immunoglobulin light chain and unknown genes coded as B613, E25, LB3(8), WE2(3), WE8-18R and WF12-18R.
在本研究中，为了筛选感染的分子生物标志物候选基因，分析了牙鲆（Paralichthys olivaceus）在感染单殖吸虫（Neoheterobothrium hirame）过程中外周血白细胞（PBL）的基因表达模式。进行了cDNA微阵列分析，以比较感染鱼和未感染鱼PBL的基因表达模式。在分析的797个基因中，有45个基因（5.6%）的表达水平发生了变化。这些基因包括特异性和非特异性免疫相关基因（基质金属蛋白酶[MMP]-9、MMP-13、白三烯B4受体、CD20受体、主要组织相容性复合体[MHC]I类、MHC II类β链、免疫球蛋白轻链和免疫球蛋白重链）。还观察到一些未知基因的显著上调和下调。选择了几个感染标记基因候选物进行进一步研究。这些基因包括MMP-9、MMP-13、白三烯b4受体、CD20受体、免疫球蛋白重链、免疫球蛋白轻链以及编码为B613、E25、LB3(8)、WE2(3)、WE8-18R和WF12-18R的未知基因。
