Mukaida Naoki, Kodama Seiji, Suzuki Keiji, Oshimura Mitsuo, Watanabe Masami
Division of Radiation Life Science, Department of Radiation Life Science and Radiation Medical Science, Kyoto University Research Reactor Institute, Osaka, Japan.
Radiat Res. 2007 Jun;167(6):675-81. doi: 10.1667/RR0835.1.
Ionizing radiation can induce chromosome instability that is transmitted over many generations after irradiation in the progeny of surviving cells, but it remains unclear why this instability can be transmitted to the progeny. To acquire knowledge about the transmissible nature of genomic instability, we transferred an irradiated human chromosome into unirradiated mouse recipient cells by microcell fusion and examined the stability of the transferred human chromosome in the microcell hybrids. The transferred chromosome was stable in all six microcell hybrids in which an unirradiated human chromosome had been introduced. In contrast, the transferred chromosome was unstable in four out of five microcell hybrids in which an irradiated human chromosome had been introduced. The aberrations included changes in the irradiated chromosome itself and rearrangements with recipient mouse chromosomes. Thus the present study demonstrates that genomic instability can be transmitted to the progeny of unirradiated cells by a chromosome exposed to ionizing radiation, implying that the instability is caused by the irradiated chromosome itself and also that the instability is induced by the nontargeted effect of radiation.
电离辐射可诱发染色体不稳定，这种不稳定在存活细胞的子代中经过多代传递，但尚不清楚为何这种不稳定能够传递给子代。为了获取有关基因组不稳定可传递性质的知识，我们通过微细胞融合将一条受照射的人类染色体转移到未受照射的小鼠受体细胞中，并检测了微细胞杂种中转移的人类染色体的稳定性。在所有引入未受照射人类染色体的六个微细胞杂种中，转移的染色体是稳定的。相比之下，在引入受照射人类染色体的五个微细胞杂种中，有四个中的转移染色体是不稳定的。畸变包括受照射染色体本身的变化以及与受体小鼠染色体的重排。因此，本研究表明，基因组不稳定可通过暴露于电离辐射的染色体传递给未受照射细胞的子代，这意味着这种不稳定是由受照射的染色体本身引起的，并且这种不稳定是由辐射的非靶向效应诱导的。
