Schnopp Christina, Rad Roland, Weidinger Anke, Weidinger Stefan, Ring Johannes, Eberlein Bernadette, Ollert Markus, Mempel Martin
Department of Dermatology and Allergy, Biederstein, Technical University Munich, Munich, Germany.
Photodermatol Photoimmunol Photomed. 2007 Apr-Jun;23(2-3):81-5. doi: 10.1111/j.1600-0781.2007.00284.x.
Regulatory T cells (T-reg cells) have been described as an important cell population in the UV treatment of inflammatory skin diseases.
We have treated five patients with generalized atopic eczema (AE) using medium-dose (15 cycles of 50 J/cm(2), total dose of 750 J/cm(2)) UVA1 therapy and have analyzed the skin-infiltrating T-cellular subsets before and after therapy. Skin biopsies were split for immunohistochemistry and Real-time PCR and analyzed for CD4, Fox-P3, GATA-3, and IL-10 transcription as well as for CD3, CD4, CD152, Fox-P3, and GITR staining.
In all the investigated patients, we observed a good clinical response to UVA1. As described previously, the number of epidermal T cells slightly declined after irradiation. However, we did not observe a general decrease in T cell numbers. Within the population of T cells, no specific difference in the kinetics of Fox-P3-positive cells and Fox-P3-specific mRNA was noted as compared with GATA-3 positive T cells. These results were paralleled by RT-PCR for IL-10 and staining for CD152, a surface molecule that has been described for T-reg cells.
In our hands, the clinical benefit of UVA1 treatment in AE patients does not seem to be due to a preferential survival/proliferation of T-reg cells.
调节性T细胞（T-reg细胞）被认为是紫外线治疗炎症性皮肤病的重要细胞群体。
我们使用中剂量（15个周期，50 J/cm²，总剂量750 J/cm²）UVA1疗法治疗了5例泛发性特应性皮炎（AE）患者，并分析了治疗前后皮肤浸润性T细胞亚群。皮肤活检组织分为两部分用于免疫组织化学和实时PCR，分析CD4、Fox-P3、GATA-3和IL-10转录以及CD3、CD4、CD152、Fox-P3和糖皮质激素诱导肿瘤坏死因子受体（GITR）染色。
在所有研究患者中，我们观察到对UVA1有良好的临床反应。如先前所述，照射后表皮T细胞数量略有下降。然而，我们未观察到T细胞数量普遍减少。在T细胞群体中，与GATA-3阳性T细胞相比，未发现Fox-P3阳性细胞和Fox-P3特异性mRNA的动力学有特异性差异。这些结果与IL-10的逆转录聚合酶链反应（RT-PCR）以及T-reg细胞表面分子CD152的染色结果一致。
在我们的研究中，UVA1治疗对AE患者的临床益处似乎并非由于T-reg细胞的优先存活/增殖。
