The effect of psoralen plus ultraviolet A in vitro in HUT-78 enhances by 5-aminolevulinic acid.
作者信息
Akita Yoichi, Watanabe Daisuke, Yanagishita Takeshi, Kuhara Tomoe, Kawamura Chiharu, Masuda Yuko, Kawada Morihiro, Nakaseko Hirono, Tamada Yasuhiko, Matsumoto Yoshinari
机构信息
Department of Dermatology, Aichi Medical University School of Medicine, Nagakute, Japan.
出版信息
Photodermatol Photoimmunol Photomed. 2007 Apr-Jun;23(2-3):95-7. doi: 10.1111/j.1600-0781.2007.00280.x.
BACKGROUND
Sezary syndrome and mycosis fungoides are forms of cutaneous T-cell lymphoma, and in the early stage of these diseases psoralen plus ultraviolet A (PUVA) is one of the treatments of choice. Photodynamic therapy using 5-aminolevulinic acid (ALA-PDT) is an effective, non-invasive, and safe treatment for most superficial skin cancers. In order to obtain greater efficacy of PUVA, we investigated the synergistic anti-tumor effects of ALA-PDT and PUVA using 8-methoxypsoralen (8-MOP) and a UVA lamp.
METHODS
The in vitro effects of PUVA and ALA-PDT and their combination in HUT-78 cell line from human SS were determined by MTT assay.
RESULTS
In our results, cell proliferation compared with controls was inhibited to 53.2% with UVA alone, 52.3% with 1 microM 8-MOP, 43.8% with 100 microM ALA, and 19.2% with combined 8-MOP and ALA.
CONCLUSION
Combined use of ALA and PUVA using 8-MOP and UVA lamps, which are widespread in Japan, had a strong anti-tumor effect in vitro. Combined treatment with ALA-PDT and PUVA using a UVA lamp appears to have a strong treatment effect.
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