Olah I, Fukumori L M I, Montanheiro P, Vergara M P, Smid J, Duarte A J S, Penalva de Oliveira A C, Casseb J
Laboratory of Allergy and Clinical Immunology - FMUSP, Institute of Infectious Diseases Emilio Ribas, São Paulo, Brazil.
Scand J Immunol. 2007 Jun;65(6):577-80. doi: 10.1111/j.1365-3083.2007.01941.x.
The present study evaluated the in vitro response to different mitogens and a candidin antigen (CMA) in Human T-cell lymphotropic virus type 1 (HTLV-1) and co-infected HIV-1/HTLV-1 patients, to identify if this co-infection may modify the spontaneous lymph proliferative response. Peripheral blood mononuclear cells from 72 healthy seronegative controls, 75 asymptomatic HTLV-1-infected carriers, 42 HAM/TSP cases, 33 solely HIV-1-infected subjects and 24 HIV-1/HTLV-1 patients were assayed in the presence and absence of mitogens (PHA, PWM and OKT3) and CMA. The HAM/TSP group had the highest proliferation rate at 3 and 6 days after culture. HAM/TSP cases showed decreased response to PHA, compared with asymptomatic HTLV-1 subjects, and most important, the co-infected HIV-1/HTLV-1 cases presented a similar response to HTLV-1-infected subjects after 3 days of culture. The singles HIV-1-infected group had decreased in vitro response. It appears that during co-infection, the HTLV-1 regulatory proteins overwhelm the action of HIV-1 regulatory proteins.
本研究评估了1型人类嗜T细胞病毒（HTLV-1）感染者以及合并感染HIV-1/HTLV-1的患者对不同促细胞分裂剂和念珠菌素抗原（CMA）的体外反应，以确定这种合并感染是否会改变自发淋巴细胞增殖反应。对72名健康血清阴性对照者、75名无症状HTLV-1感染携带者、42例HAM/TSP患者、33名单纯HIV-1感染受试者以及24例HIV-1/HTLV-1患者的外周血单个核细胞，在有和没有促细胞分裂剂（PHA、PWM和OKT3）及CMA的情况下进行检测。HAM/TSP组在培养3天和6天后增殖率最高。与无症状HTLV-1感染者相比，HAM/TSP患者对PHA的反应降低，最重要的是，合并感染HIV-1/HTLV-1的患者在培养3天后呈现出与HTLV-1感染受试者相似的反应。单纯HIV-1感染组的体外反应降低。似乎在合并感染期间，HTLV-1调节蛋白压倒了HIV-1调节蛋白的作用。
